质谱法对鲍曼不动杆菌的快速鉴定研究  

Research of rapid mass spectrum identification of Acinetobacter baumannii

作　　者：杭亚平[1] 胡龙华[1] 汪红[1] 宁长秀[1] 钟桥石[1] 胡晓彦[1] 章白苓[1] 张楠[1] 王小中[1] 

机构地区：[1]南昌大学第二附属医院检验科,南昌330006

出　　处：《中国临床药理学杂志》2015年第2期110-112,共3页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金资助项目(81460327)

摘　　要：目的建立表面解吸常压化学电离质谱(SDAPCI-MS)技术检测鲍曼不动杆菌特有的挥发性代谢产物(VCs)以便快速鉴定鲍曼不动杆菌。方法复苏临床分离获得的鲍曼不动杆菌10株,接种到血琼脂平板上,37℃孵育培养,用SDAPCI-MS对培养不同时间的鲍曼不动杆菌进行VCs检测。结果与空白培养基比较,检测到鲍曼不动杆菌特有的VCs及特征的质荷比峰(m/z 60和118),且鲍曼不动杆菌在不同培养时间产生的VCs基本相同。结论 SDAPCI-MS有望通过特征性VCs对鲍曼不动杆菌进行快速检测。Objective To establish the rapid identification of Acineto-bacter baumannii by detecting specific volatile metabolites of Acinetobacter baumannii through surface desorption atmospheric pressure chemical ioni-zation mass spectrometry （ SDAPCI-MS）.Methods Ten clinical isola-ted Acinetobacter baumannii were resuscitated and vaccinated to blood agar plates at 37 ℃incubation and culturing.SDAPCI-MS was adopted to detect volatile metabolites of Acinetobacter baumannii by different cul-turing time.Results Compared with blank culture medium , the pecu-liar volatile metabolites were detected ,the characteristic peaks are mass -to-charge ratio 60 and 118.Basically the same volatile metabolites of Acinetobacter baumannii were generated by different culturing time.Con-clusion The rapid identification of Acinetobacter baumannii through peculiar volatile metabolites is hopefully applied by SDAPCI -MS.

关 键 词：鲍曼不动杆菌 挥发性代谢产物 表面解吸常压化学电离质谱 

分 类 号：R978.1[医药卫生—药品] R372[医药卫生—药学]