基于Fe_3O_4-PEI纳米粒子构建葡萄糖传感器的研究  

Glucose sensor based on Fe_3O_4-PEI nanoparticles

作　　者：何传新[1] 任圣颖 谢敏随 袁安朋[1] 洪飞[1] 张黔玲[1] 刘剑洪[1] 

机构地区：[1]深圳大学化学与化工学院,深圳518060

出　　处：《深圳大学学报（理工版）》2015年第1期76-81,共6页Journal of Shenzhen University(Science and Engineering)

基　　金：国家自然科学基金资助项目(21374064;21004040)~~

摘　　要：采用共沉淀法制备核层为四氧化三铁(Fe3O4)壳层为聚乙烯亚胺(polyethyleneimine,PEI)的磁性复合纳米粒子Fe3O4-PEI.扫描电子显微镜和透射电子显微镜表征结果显示,制备的磁性复合纳米粒子Fe3O4-PEI粒径均匀,直径约为25 nm.通过振动样品磁强计比较Fe3O4-PEI和Fe3O4纳米粒子的磁滞回线,结果表明,经PEI包覆后复合纳米粒子饱和磁化值为38.2 emu/g,仍具有较好的磁性.热重分析表明,包覆在Fe3O4纳米粒子表面的PEI质量分数约为23.26%.通过静电作用,实现了Fe3O4-PEI复合纳米粒子对葡萄糖氧化酶的负载,以铂电极为基底电极,制备了Fe3O4-PEI-GOx/Pt葡萄糖传感器.在最优测试条件下,该修饰电极对葡萄糖表现出优异的电化学催化性能,具有灵敏度高、抗干扰能力强、稳定性好的特点.Fe3O4-PEI nanoparticles with Fe3O4 core and polyethylenimine （PEI） shell were prepared via the co-precipitation method. Results from scanning electron microscope （ SEM ） and transmission electron microscope （ TEM） show that Fe3 O4-PEI nanoparticles present globular shape with almost uniform diameters of about 25 nm. Magnetic hysteresis loops of Fe3 O4-PEI and Fe3 O4 nanoparticles were obtained by a vibrating sample magnetometer （ VSM） . The magnetism of Fe3 O4 nanoparticles still remains a lot after coating with PEI, and the saturation magneti-zation value of Fe3 O4-PEI nanoparticles is 38. 2 emu/g. Thermogravimetry （ TG） analysis indicats that the loading amount of PEI on the surface of Fe3 O4 nanoparticles is about 23. 26%. The glucose oxidase （ GOx） enzyme was im-mobilized on the Fe3 O4-PEI nanoparticles surface by electrostatic interaction. Fe3 O4-PEI-GOx/Pt glucose sensor was prepared with platinum （ Pt） as its basal electrode, which exhibits a good electrochemical catalysis for glucose, high sensitivity, strong anti-interference ability and long-term durability for glucose detection under an optimal preparation condition.

关 键 词：高分子化学 葡萄糖传感器 磁性纳米粒子 聚乙烯亚胺 葡萄糖氧化酶 静电作用 

分 类 号：O63[理学—高分子化学]