CLCN3/MMTV-PyMT转基因小鼠杂交群体的建立及鉴定  

Establishment and identification of a ClC-3/MMTV-PyMT hybrid mouse strain

作　　者：邓璐璐[1] 李琴 吴慧[1] 毛建文[1] 徐彬[3] 

机构地区：[1]广东药学院基础学院,广州510006 [2]贵州省六盘水市人民医院,贵州六盘水553001 [3]广东药学院生命科学与生物制药学院,广州510006

出　　处：《中国实验动物学报》2014年第6期17-21,I0010,共6页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金项目(编号:31371144)

摘　　要：目的建立并鉴定CLCN3/MMTV-Py MT双转基因小鼠杂交群体,构建氯通道Cl C-3过表达的自发乳腺肿瘤转基因小鼠模型。方法将CLCN3转基因小鼠和MMTV-Py MT转基因小鼠分别进行繁殖,选取阳性子代鼠进行配对,培育杂交后代,然后通过PCR鉴定基因型、通过组织免疫荧光方法、Western blot检测转基因小鼠蛋白表达情况。结果成功繁育CLCN3转基因小鼠和MMTV-Py MT转基因小鼠,经PCR鉴定成功构建CLCN3/MMTV-Py MT双转基因鼠杂交群体,并成功保种和扩群,经组织免疫荧光和Western-blot分析双转基因小鼠的Cl C-3蛋白的表达高于MMTV-Py MT转基因小鼠。结论成功构建Cl C-3过表达的自发乳腺肿瘤转基因小鼠模型,为进一步研究Cl C-3在肿瘤生长和转移中的功能提供了良好的动物模型。Objective To establish a CLCN3/MMTV-PyMT double transgenic mouse model of spontaneous breast tumor with simultaneously overexpressing ClC-3.Method CLCN3 transgenic mice were crossed with MMTV-PyMT spon-taneous mammary tumor model mice.The genotype was determined by PCR.The expression of ClC-3 in tissues was detected by immunofluorescence and Western blot.Results CLCN3 and MMTV-PyMT transgenic mice were bred and CLCN3/MMTV-PyMT hybrid mouse model was successfully established.The ClC-3 expression in CLCN3/MMTV-PyMT hybrid mice was higher than that in the MMTV-PyMT mice, assessed by immunofluorescence and Western blot analysis.Conclu-sions Transgenic mouse models of spontaneous breast cancer with simultaneously overexpressing ClC-3 are successfully established.The double transgenic mice provide a good animal model for further research of ClC-3 in tumor growth and metastasis.

关 键 词：CLCN3 MMTV-Py MT 转基因小鼠 乳腺癌 

分 类 号：Q95-33[生物学—动物学]