地鳖多肽提取物的抗氧化衰老机制  

Mechanism of polypeptide extracts of Eupolyphaga sinensis Walker against oxidative aging in mice

作　　者：谷崇高 张永红[1] 白若雨 田美杰[1] 沈红[1] 

机构地区：[1]北京农学院动物科学技术学院,兽医学(中医药)北京市重点实验室,北京102206

出　　处：《中国实验动物学报》2014年第6期66-74,I0014,共10页Acta Laboratorium Animalis Scientia Sinica

基　　金：北京市教委项目(PXM2013014207000067和KM201410020005);北京农学院科技创新团队科研能力提升工程项目(KCT2014012);2014北京市本科生研究训练项目

摘　　要：目的探讨地鳖多肽(ESW polypeptide)提取物抗氧化衰老机制的研究。方法小鼠连续腹腔注射D-半乳糖20 d,建立衰老模型,同时小鼠灌服不同剂量地鳖多肽提取物每日(0、40、80、160 mg/kg),观察小鼠的正常活动、运动和耐应激能力。分别采用黄嘌呤氧化酶法、分光光度法检测小鼠血液和不同组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-PX)活力,以及丙二醛(MDA)和还原型谷胱甘肽(GSH)含量;免疫荧光法检测细胞核转录因子2(Nrf2)在Caco-2细胞的表达。结果与对照和多肽组比较,衰老组小鼠体重增重缓慢、组织脏器系数降低、运动时间缩短、抗应激能力降低、组织中抗氧化酶活力降低。随着地鳖多肽剂量增加,多肽组小鼠体重增加明显,肝脏、脾脏和肾脏指数增加显著,小鼠静力和动力运动时间明显延长,小鼠耐缺氧、耐高温和运动时间延长并接近对照组,血液和不同组织中的SOD、CAT、GSH-PX活力及GSH含量显著提高,但MDA含量降低。与对照组比较,地鳖多肽组Caco-2细胞核内Nrf2表达量明显增加,接近阳性对照组。结论地鳖多肽可能通过启动Nrf2-ARE抗氧化信号通路,提高D-半乳糖致衰老小鼠抗应激和抗氧化能力,从而延缓小鼠氧化衰老。Objective The purpose of this study was to investigate the mechanism of action of polypeptide extracts of Eupolyphaga sinensis Walker （ ESW） against oxidative aging.Methods Mice were intraperitoneally injected D-galac-tose for consecutive 20 days to establish an aging mouse model.The model mice were administered with different doses of ESW polypeptide （0, 40, 80, 160 mg/kg/d）.The normal activity, movement and anti-stress ability of the mice were observed.The activity of superoxide dismutase （SOD）, catalase （CAT）, glutathione peroxidase （GSH-PX） in blood and different tissues and the content of glutathione （ GSH） and malondialdehyde （ MDA） of the aging mice were assessed by xanthin oxidase activity measurement and spectrophotometry, respectively.The expression of nuclear factor erythroid 2-related factor 2 （Nrf2） in Caco-2 cells was detected by immunofluorescence.Results Comparing the control and polypeptide groups, there were significant decreases of body weight gain, organ indexes, anti-stress ability and activity capacity, the activity of SOD, CAT, GSH-PX and the content of GSH, and an increase of the content of MDA in blood and different tissues in the aging mice.With the increasing dose of polypeptide extracts of ESW, the body weight gain, organ indexes of the liver, spleen and kidney were significantly increased, the static and dynamic exercise time was prolonged in the poly-peptide group, and their abilities of hypoxia tolerance and heat tolerance were close to that of normal controls.The SOD, CAT, GSH-PX activity and GSH level in blood and different tissues were significantly increased, but MDA content decreased.The expression of Nrf2 in Caco-2 cell nuclei was significantly increased in the polypeptide group, close to that of the positive control group.Conclusions The results of our study show that polypeptide extracts of ESW improve the anti-stress and antioxidative capacity in D-galactose-induced mouse models of oxidative aging by initiating Nrf2-ARE antioxidant signaling

关 键 词：地鳖多肽 D-半乳糖 衰老模型鼠 抗氧化作用 细胞核转录因子2 

分 类 号：Q516[生物学—生物化学]