卵巢组织玻璃化冷冻的研究进展  

Research progress of ovarian tissue vitrification

作　　者：王劲松[1] 左琴[1] 范涛[1] 李保文[1] 

机构地区：[1]中国食品药品检定研究院,北京100050

出　　处：《中国比较医学杂志》2014年第12期71-74,81,共5页Chinese Journal of Comparative Medicine

基　　金：国家科技部支撑课题项目(2013BAK11B00)

摘　　要：玻璃化冷冻法是在急速降温过程中使溶液行成一种无冰晶的玻璃化状态,可以最大程度避免冰晶形成对细胞膜及细胞骨架结构的损伤。影响卵巢组织玻璃化冷冻保存效果的主要因素有卵巢组织块的大小、冷冻保护剂的种类、渗透平衡方法、冷冻载体等。卵巢组织结构较复杂,尚未形成统一的玻璃化冷冻程序。提出了目前存在的问题。Vitrification provides a rapid cooling to induce glass-like solidification inside cells and protect cell membrane and cytoskeleton system free of injury by ice crystals.The main factors that can influence the effect of vitrification are the size of ovarian tissue, the kind of cryoprotectants, the method of permeation, carrier system and so on. Ovarian tissue structure is very complex, and there is no uniform standardized protocol of vitrification yet.This paper presents also the current problems of ovarian tissue vitrification.

关 键 词：卵巢 玻璃化冷冻 影响因素 

分 类 号：R33[医药卫生—人体生理学]