上转发光免疫层析法检测乙肝病毒外膜大蛋白在不同类型慢性肝病中的临床意义  

The clinical significance of up-converting phosphor immunochromatography in detecting hepatitis B virus large envelope protein in various types of chronic liver disease

作　　者：王妙婵[1] 过建春 王青青[1] 徐爱芳 

机构地区：[1]浙江大学免疫研究所,杭州310031 [2]杭州市西溪医院

出　　处：《中华实验和临床病毒学杂志》2014年第6期452-454,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的 探讨上转发光免疫层析法检测的乙肝病毒外膜大蛋白（HBV-LP）在慢性乙型肝炎（CHB）、乙肝后肝硬化（LC）、乙肝原发性肝癌（PHCC）患者血清中水平变化及临床意义.方法 利用一种新的基于上转发光法的免疫层析检测技术（UPT）检测260例慢性乙型肝炎、190例乙肝肝硬化及45例乙肝原发性肝癌患者血清中乙肝病毒外膜大蛋白（HBV-LP）的含量,化学发光法检测乙肝E抗原（HBeAg）含量,实时荧光定量PCR法检测乙肝病毒核酸（HBV DNA）.结果 HBV-LP阳性结果浓度水平CHB组主要以＞40 U/ml的高浓度为主,LC组和PHCC组主要集中在＜10 U/ml的低浓度范围内;HBeAg、HBV DNA和HBV-LP的阳性率均表现为CHB组＞LC组＞PHCC组,HBeAg和HBV DNA在LC和PHCC患者中的阳性率均明显低于CHB患者,差异有统计学意义（P＜0.01）;HBV-LP在LC和PHCC患者中的阳性率明显高于HBV DNA和HBeAg,差异有统计学意义（P＜0.05）.结论 上转发光免疫层析法检测HBV-LP水平快捷可靠并且对慢性肝病进展的诊断以及疗效监测有重要的价值.Objective To explore the clinical significance of up-converting phosphor immunochromatography in detecting hepatitis B virus large envelope protein （HBV-LP） in serum of chronic hepatitis B （CHB）,liver cirrhosis （LC） and primary hepatic carcinoma （PHCC） patients.Methods A new UPT-based immunochromatographic technology was employed to detect hepatitis B virus large envelope protein （HBV-LP） in serum of 260 CHB,190 LC and 45 PHCC patients; chemiluminescence was adopted to detect HBeAg; real-time fluorescent quantitative PCR was utilized to detect hepatitis B virus DNA （HBV DNA）.Results The majority of CHB patients have high concentrations of HBV-LP （〉40 U/ml） ; most LC and PHCC patients have low concentrations of HBV-LP （〈 10 U/ml） ; CHB patients,LC patients and PHCC patients are all ranked in descending order in terms of the positive rates of HBeAg,HBV DNA and HBV-LP; the positive rates of HBeAg and HBV DNA in LC and PHCC patients are lower than those in CHB patients and the difference is statistically significant （P 〈 0.01）; within the groups of LC and PHCC patients,the positive rate of HBV-LP is significantly higher than those of HBV DNA and HBeAg and the difference is statistically significant （P 〈 0.05）.Conclusion As a swift and reliable method in detecting HBV-LP levels,up-converting phosphor immunochromatography is of great value in diagnosing chronic liver disease and monitoring treatment effects.

关 键 词：病毒包膜蛋白质类 肝炎 乙型 肝硬化 肝肿瘤 

分 类 号：R446.6[医药卫生—诊断学]