青龙衣水解单宁含量测定方法研究  

Determination methods of hydrolysable tannins fromJuglans regia L.

作　　者：姜蕊[1] 张霞[1] 邢丹[1] 刘子祯[1] 刘伟锐[1] 杨悦[1] 王晓宏[1] 折改梅[1] 

机构地区：[1]北京中医药大学中药学院中药化学系,北京100102

出　　处：《西北药学杂志》2015年第1期16-20,共5页Northwest Pharmaceutical Journal

基　　金：北京中医药大学自主课题(编号:2014-JYBZZ-XS-119和2013-JYBZZ-JS-137);国家级大学生创新创业计划(编号:201210026042);北京市共建项目(编号:BJGJ1326)

摘　　要：目的建立青龙衣水解单宁含量测定的方法。方法以没食子酸为对照品,研究青龙衣水解单宁的含量测定,采用紫外-可见分光光度法,确定改良的Folin-Ciocalteu法运用于青龙衣单宁含量测定显色的最佳条件,测定青龙衣酸解前后总单宁的含量。结果测定没食子酸的标准曲线方程为：Y=151.8 X＋0.041,在0.992~4.960μg·mL^-1范围内线性关系良好,r=0.999（n=6）。优化的显色方法为1.0mL的样品液,Folin-Ciocalteu 1.5 mL,加去离子水10 mL,用70g·L^-1的碳酸钠定容至25mL,30℃显色1.5h。结论该显色方法操作简便,准确度高、稳定性和重复性良好,既适用于未酸解单宁样品的含量测定,也适用于酸解后单宁样品的含量测定,对水解单宁类成分的含量测定具有普遍适用性。Objective To establish a method for the determination of the hydrolyzed tannins from Juglans regia L.. Methods Gallic acid as a reference compound. UVspectrophotometry was used for the determination of the hydrolyzed tannins from Juglans regia L.. The modified Folin-Ciocalteu method was set up, then it was used to determine the contents of both the un-hydrolyzed tannins and the hydrolyzed tannins. Results The standard curve equation was： Y= 151.8X＋0. 041, and gallic acid was linear in the range of 0. 992-4. 960 μg · mL^-1 , r=0. 999（n=6）. The optimal conditions for determining the hydrolyzed tannins from Juglans regia L. were as followed： 1.0 mL of the sample, 1.5 mL of F-C reagent, 10 mL of water, with 70 g · L^-1 sodium carbonate solution to the volume, treatment at 30 ℃ for 1.5 h. Conclusion This method is simple, accurate, stable and repeatable. It has universal applicability for the determination of both the un-hydrolyzed tannins and the hydrolyzed tannins.

关 键 词：青龙衣 单宁 酸解 紫外-可见分光光度法 

分 类 号：R282[医药卫生—中药学]