新疆地区汉族人群转录因子7类似物2基因多态性与2型糖尿病的相关性研究  

Relationship between Transcription Factor 7- like 2 Gene and Type 2 Diabetes Mellitus among Han Population in Xinjiang

作　　者：曹冰[1] 朱筠[1] 张明琛[1] 

机构地区：[1]新疆医科大学第一附属医院内分泌科,新疆乌鲁木齐市830054

出　　处：《中国全科医学》2015年第3期263-268,共6页Chinese General Practice

基　　金：新疆维吾尔自治区自然科学基金资助项目(2012211A067)

摘　　要：目的探讨新疆地区汉族人群转录因子7类似物2(TCF7L2)基因rs11196218、rs10749127、rs290487、rs290481多态性与2型糖尿病(T2DM)的相关性。方法选取2013年11月—2014年2月新疆医科大学第一附属医院收治的汉族T2DM患者164例为T2DM组,另选取同时期体检的健康人群195例为对照组,测量并记录身高、体质量、腹围、血压,检测血糖、糖化血红蛋白(Hb A1c)、胰岛素、C肽、血脂、尿酸水平。提取外周血DNA,检测TCF7L2基因rs11196218、rs10749127、rs290487、rs290481基因型及等位基因频率。多因素Logistic回归分析T2DM的影响因素。结果对照组与T2DM组收缩压(SBP)比较,差异无统计学意义(P>0.05);T2DM组患者舒张压(DBP)、三酰甘油(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、空腹血糖及Hb A1c较对照组升高,高密度脂蛋白胆固醇(HDL-C)较对照组降低(P<0.05)。对照组与T2DM组rs11196218、rs290481基因型和等位基因频率分布,差异均无统计学意义(P>0.05);两组rs10749127 TT基因型分布,差异有统计学意义(P<0.05),rs290487 CC基因型分布及等位基因频率分布,差异有统计学意义(P<0.05)。rs10749127的CC、CT、TT基因型T2DM患者间空腹血糖及尿酸水平比较,差异有统计学意义(P<0.05)。rs290487的CC、CT、TT基因型间各指标比较,差异均无统计学意义(P>0.05)。多因素Logistic回归分析结果显示:rs290487CC基因型、腰围、体质指数(BMI)、空腹血糖及Hb A1c与T2DM有回归关系(P<0.05)。结论 TCF7L2基因rs10749127、rs290487与新疆地区汉族人群的T2DM的发病率有关,未发现rs11196218、rs290481与T2DM发病的关联。Objective To investigate the relationship between polymorphisms of transcription factor 7 - like - 2 （TCF7L2） gene （ rs11196218,rs10749127,rs290487,rs290481 ） and type 2 diabetes mellitus （ T2DM ） among Han population in Xinjiang. Methods 164 Han patients with T2DM who were treated in the First Affiliated Hospital of Xinjiang Medical University from November 2013 to February 2014,were included in T2DM group,at the same time,195 healthy people who received physical examination were included in control group. The height,body mass,abdominal circumference,blood pressure,levels of blood glucose,glycated hemoglobin（ HbA1c ）,insulin,C - peptide,blood lipids and uric acid were measured and recorded. Peripheral blood DNA was extracted,genotype and allele frequency were detected for TCF7L2 gene rs11196218,rs10749127, rs290487, rs290481. The influence factors for T2DM were analysed by multivariable Logistic regression. Results There was no significant difference in systolic blood pressure（SBP）between control group and T2DM group （P ﹥ 0. 05）. The diastolic blood pressure（DBP）,levels of three acyl glycerin（TG）,total cholesterol（TC）,low - density lipoprotein cholesterol（LDL - C）,fasting blood glucose and HbA1c of patients in T2DM group were significantly higher than those of patients in control group,but the high density lipoprotein cholesterol（HDL - C）level of patients in T2DM group was significantly lower than that of patients in control group（P ﹤ 0. 05）. There was no significant difference in genotype and allele frequency distribution of rs11196218 and rs290481 between the control group and T2DM group（ P ﹥ 0. 05 ） . There was significant difference in distribution of rs10749127 TT genotype between control group and T2DM group（P ﹤ 0. 05）. There were significant differences in genotype and allele frequency distribution of rs290487 CC between control group and T2DM group（P ﹤0. 05）. There were significant differences in levels of fasting glucose and uric acid

关 键 词：糖尿病 2 型 转录因子 7 类似物 2 基因 多态性 单核苷酸 

分 类 号：R587.1[医药卫生—内分泌]