多胎和单胎山羊品种Bcl-2和Bax基因的克隆及组织表达研究  

Cloning and tissue expression of Bcl-2 and Bax genes in high and low prolific goat breeds

作　　者：胡亮[1] 字向东[1] 卢建远[1] 马力[1] 熊显荣[1] 王永[1] 任称罗尔日 任陶 

机构地区：[1]西南民族大学生命科学与技术学院,四川成都610041 [2]四川省理县畜牧局,四川理县623100

出　　处：《畜牧与兽医》2015年第1期28-32,共5页Animal Husbandry & Veterinary Medicine

基　　金：四川省应用基础项目(2013JY0043)

摘　　要：以低繁藏山羊和高繁金堂黑山羊卵巢等组织为试验材料,通过RT-PCR,对Bcl-2和Bax基因c DNA进行克隆、序列分析,并采用Real-time PCR对其在发情前期母羊垂体、卵巢、子宫和输卵管等组织的表达进行检测。结果:山羊Bcl-2基因编码区全长为690 bp,共编码229个氨基酸,2个品种间有3处碱基差异,但未导致氨基酸的差异;Bax基因编码区全长为579 bp,共编码192个氨基酸,2个品种的同源性为100%。Bcl-2和Bax mRNA在2个品种山羊的卵巢、子宫、输卵管、垂体中均有表达,但品种间差异不显著(P>0.05)。说明Bcl-2和Bax基因在动物进化中比较保守,与山羊多羔性状的相关性有待进一步研究。This study was carried out to obtain and analyze sequences of Bcl-2 and Bax cDNAs of nonprolific Tibetan goat and prolific Jin- tang black goat by RT-PCR, and determine their mRNA expressions in the pituitary, ovary, uterus and oviduct at proestrus by real-time PCR. The result showed that the coding region of goat Bcl-2 gene was 690 bp long, encoding 229 amino acids. There were three base chan- ges between these two breeds, but it did not result in mutation in the deduced amino acid sequence. The coding region of goat Bax gene was 579 bp long, encoding 192 amino acids. The Bcl-2 and Bax mRNA were expressed in pituitary, ovary, uterus and oviduct in both these two breeds of the goat, but there was no significant difference between breeds （P〉0.05）. The result showed that both Bcl-2 and Bax genes were conservative in the course of animal evolution, and the effect on prolificacy in goats required further investigation.

关 键 词：藏山羊 金堂黑山羊 Bcl-2 BAX 克隆 q PCR 

分 类 号：S814[农业科学—畜牧学]