布鲁氏菌病疫苗(S2株)免疫羊抗体消长规律的研究  

Studies on the Antibody Development in Sheep and Goats Immunized with Brucellosis Vaccine (S2)

作　　者：申捷 宝音达来 马立峰 戴晓光 马晓雪 苏胜杰 萨茹拉 云涛 双燕 郝璐 郭金贵 菊花 刘志伟 毛开荣[2] 申之义[3] 赵心力 

机构地区：[1]内蒙古自治区动物疫病预防控制中心,内蒙古呼和浩特市010010 [2]中国兽医药品监察所,北京100081 [3]内蒙古农业大学,内蒙古呼和浩特市010018

出　　处：《中国动物检疫》2015年第1期68-73,共6页China Animal Health Inspection

摘　　要：为探讨羊经布鲁氏菌病疫苗免疫后的血清抗体消长规律,本研究分别对绵羊和山羊进行布鲁氏菌S2株疫苗不同剂量的灌服,于免疫前和免疫后不同时间采集血清样品,采用虎红平板凝集试验和试管凝集试验进行血清抗体检测。结果表明,绵羊及山羊均在免疫后20d时血清抗体阳性率达到最高峰,之后呈下降趋势,免疫后90天降至低点。免疫后90至360d,绵羊组抗体阳性率几乎一直维持在14%以下,各时段无显著性差异(P>0.05);山羊组抗体阳性率在免疫后90至360d期间时高时低,起伏较大,无明显规律。试验期间,山羊的抗体阳性率普遍高于绵羊,且降升幅度较大。同时,研究表明,同种羊100亿和200亿两个免疫剂量组的抗体阳性率差异不大。To investigate the pattern of increasing or decreasing of serum antibody in sheep and goats immunized with brucellosis vaccine,RPST and tube agglutination test were used in the study to determine the level of serum antibody in sheep and goats before and after immunization with Brucella strain S2 vaccine. The results indicated that the serum antibody positive rate in sheep reached the highest level 20 DPI,then started to decrease and remained relatively low level 90-360 DPI,mostly below 6%,and relatively higher in few individuals,up to 14%. The positive rate in goats reached the highest 20 DPI,then decreased and remained low or relatively low level 90-360 DPI,but with decreasing or increasing sometimes. Comparatively,antibody positive rate in goats was generally higher than in sheep,and the descending and ascending range was bigger. It was also shown that there was no significant difference in antibody positive rate between 10 billion CFU dose group and 20 billion CFU dose group in same species.

关 键 词：绵羊 山羊 布鲁氏菌病 免疫 抗体监测 

分 类 号：S858.26[农业科学—临床兽医学]