拟南芥花粉发育相关突变体gpl1的表型分析及遗传定位  

Phenotypes Analysis and Genetic Positioning of Arabidopsis thaliana Mutant gpl1 Related to Pollen Development

作　　者：孙恒吉[1] 司英语[1] 王伟倩[1] 陈琰爽 周树敏[1] 张卫[1] 

机构地区：[1]上海大学能源作物育种及应用重点实验室,上海200444

出　　处：《河南大学学报（自然科学版）》2015年第1期68-72,共5页Journal of Henan University:Natural Science

基　　金：国家自然科学基金资助(D.09-0112-08-402)

摘　　要：以拟南芥(Arabidopsis thaliana)Col生态型种子为材料,经EMS诱变获得一株后代表型分离比异常的突变体gpl1(group less 1,gpl1),进一步观察发现,其果荚短小萎缩,不能产生种子,花粉败育,成熟花粉粒无细胞核.另外,F1代的表型分析显示gpl1突变体的性状是受隐性基因控制,而F2代的遗传分离比分析显示gpl1突变体不遵循经典的遗传分离比定律.对gpl1基因进行图位克隆,并将其定位于2号染色体上T9J22与F12C20两个分子标记之间202kb的区段内.Through EMS mutagenesis,the author obtained an Arabidopsis thaliana male sterile mutant gpl1 resulting in abnormal separation ratio,showing the reduced and unextended silique without seed and the sterile pollen without nuclear in microgainetophyte.In addition,phenotypes analysis of heterozygote F1 generation showed that the mutant was controlled by a recessive nuclear gene,however genetic separation ratio analysis of F2 generation showed that the mutant did not follow the classical genetic segregation ratio.The gpl1 gene was mapped to a region of 202 kb between the molecular makers T9J22 and F12C20 on chromosome 2 using map based cloning technique.

关 键 词：gpl1 拟南芥 图位克隆 雄性不育 

分 类 号：Q344[生物学—遗传学] Q94