麦洼牦牛NOBOX基因克隆与序列分析  

Cloning and Sequence Analysis of NOBOX Genes in Yak

作　　者：李博浩[1] 符梅[1] 兰道亮[2] 熊显荣[1] 李键[1,2] 

机构地区：[1]西南民族大学生命科学与技术学院,四川成都610041 [2]西南民族大学青藏高原研究院,四川成都610041

出　　处：《动物医学进展》2015年第2期39-43,共5页Progress In Veterinary Medicine

基　　金：西南民族大学中央高校基本科研业务费专项资金项目(14ZYXS54)

摘　　要：为了解牦牛NOBOX基因的特点,根据GenBank中公布的牛NOBOX基因序列设计2对引物,分两段扩增麦洼牦牛的NOBOX基因,然后测序并拼接。序列分析表明,扩增的牦牛NOBOX基因大小为1 975bp,其中开放阅读框全长1 500bp,编码499个氨基酸,分子质量为53.12ku。核苷酸序列同源性分析表明,NOBOX基因具有相对较高的保守性,牦牛与牛的NOBOX基因核苷酸序列同源性很高,为97%。在此基础上,借助Mega 5.0软件,采用N-J算法构建了NOBOX氨基酸的系统进化树,分析了不同物种间的进化关系。牦牛NOBOX基因序列的成功克隆,为麦洼牦牛卵母细胞成熟及早期胚胎发育分子机制的研究及麦洼牦牛的遗传资源保护和育种提供了理论基础。To understand the character of NOBOX genes of yak,two pairs of primers were designed according to the published NOBOX gene sequence of Bos taurus in GenBank. The NOBOX gene of yak was amplified and sequenced. The results of sequence analysis show that the length of the cloning NOBOX cDNA is 1 975 bp,containing I 500 bp open reading frame （ORF）. It encodes a peptide of 499 amino acids whose molecular mass is 53. 12 ku. Using sequence homology analysis, NOBOX gene is more conservative, the gene sequences of yak and Bos taurus have high homology which is 97.0 %. On this basis, the experiment used Mega 5.0 software with NJ algorithm to construct a phylogenetic tree of NOBOX protein, analyzed the evolutionary relationships among different species. The successful cloning and sequence of NOBOX gene of yak provide important basis for the study of the molecular mechanism of Maiwa yak oocyte maturation and its early embryonic development, as well as the protection of genetic resources and breeding.

关 键 词：克隆 序列分析 NOBOX基因 牦牛 

分 类 号：S852.21[农业科学—基础兽医学] Q785[农业科学—兽医学]