自制中药复方片剂对小鼠的急性毒性试验  

Acute Toxicity Test of Self-made Traditional Chinese Herbal Compound Tablet in Mice

作　　者：王静[1] 蒋万[1] 何生虎[1] 李志红[1] 葛松[1] 郭澍强 

机构地区：[1]宁夏大学农学院,宁夏银川750021

出　　处：《动物医学进展》2015年第2期120-124,共5页Progress In Veterinary Medicine

基　　金：宁夏科技攻关项目[(C)kjnx2009-3]

摘　　要：为了验证自制中药复方片剂的安全性,确定中草药配伍提取后制成的片剂毒性,采用小鼠急性毒性试验方法测定小鼠半数致死量（LD50）。结果表明,复方中药片剂对小鼠腹腔的LD50=42 884mg/kg,其Dmin=10 000 mg/kg,Dmax=80 000 mg/kg,MLD=16 822 mg/kg,LD50的95%可信限为35 222.77-50 545.23mg/kg。注射剂量为47 604mg/kg的小鼠,其肝脏肿大,边缘呈暗红色,易碎,肾脏肿大不明显;而61 742mg/kg与80 079mg/kg的小鼠肝脏和肾脏均肿大,肝脏表面呈现紫红色,其切面呈暗红色,质地软易碎,肠系膜略有充血现象,其他脏器无眼观变化。根据毒理学评价标准,LD50〉10 000mg/kg,属于无毒性物质,本试验证实中药复方片剂对小鼠安全无毒。In order to evaluate the safety and ascertain the toxicity of the self-made traditional Chinese herbal compound tablet, the experiment of acute toxicity and the determination of LD50 of self-made traditional Chinese herbal compound tablet were conducted in mice with different doses. The result showed that the LD50 of traditional Chinese herbal compound tablet was 42 884 mg per kg weight, the minimal dose was 10 000 mg per kg weigh,the maximal dose was 80 000 mg per kg weight, the MLD was 16 822 mg per kg weight and the 95% confidence interval was from 35 222.77 to 50 545.23 mg per kg weight. Dissected lesions of died mice showed that the liver in the dose of 47 604 mg per kg weight was enlarged and fragiler with dark red edge, but the kindney＇s pathological lesions were not obvious. However, the liver and kindney in the doses from 61 742 to 80 079 mg per kg weight were enlarged and their surface were purple, the mesentery bleeding slightly,but the other organs didn＇t show obvious pathological lesions. According to the toxicological evaluation standard, when LD50 is greater than 10 000 mg per kg weight, the medicine belongs to the non-toxic matter, the results confirmed that the self-made traditional Chinese herbal compound tablet was safe and non-toxic in mice.

关 键 词：急性毒性 腹腔注射 半数致死量 

分 类 号：S856.9[农业科学—临床兽医学] S853.76[农业科学—兽医学]