焦磷酸测序技术检测大豆过敏原成分GlymBd 30K基因  

The Detection of Allergen Glym Bd 30K in Soybean by Pyrosequencing Technology

作　　者：金莹[1] 房保海[2] 刘新亮 孙涛[2] 贾俊涛[2] 赵丽青[2] 孙雯娴[2] 

机构地区：[1]黄岛出入境检验检疫局,山东青岛266555 [2]山东出入境检验检疫局技术中心,山东青岛266002 [3]黑龙江出入境检验检疫局,黑龙江哈尔滨150001

出　　处：《安徽农业科学》2015年第3期29-31,49,共4页Journal of Anhui Agricultural Sciences

基　　金：国家质检总局项目(2011IK221)

摘　　要：[目的]建立一种通过焦磷酸测序技术检测大豆过敏原成分的方法。[方法]针对大豆Glym Bd30K基因设计特异性PCR引物和焦磷酸测序引物,利用设计的特异性引物扩增各测试材料的特异基因片段并进行焦磷酸测序。[结果]该检测方法对大豆过敏原成分具有非常好的重现性和特异性,测序结果在Gen Bank中进行同源性比对分析,表明目标序列能够作为鉴定Glym Bd 30K基因很好的序列。[结论]为食品中的大豆过敏原成分快速检测提供很好的技术支撑。[Objective]To establish a rapid method to detect soybean allergens by pyrosequencing technology. [Method] The conserved sequences for Glym Bd30 K gene specific PCR amplification,and then using pyrosequencing technology for PCR amplification products. Using primers design specific gene fragments in tested materials were amplified,and then pyrosequencing. [Result]The results had very good reproducibility; the sequencing results were homology found in Gen Bank. The target sequence to sequence as the identification of Glym Bd 30 K gene was very good.[Conclusions] A simultaneous quantitative detection of 96 samples by pyrosequencing rapid detection methods was Successfully established,it will provide good technical support for rapid detection of allergens for export soybeans and their products.

关 键 词：焦磷酸测序 大豆 过敏原 PCR 

分 类 号：S188[农业科学—农业基础科学]