高体革鯻温和气单胞菌的分离·鉴定及药敏试验  

Isolation and Identification of Pathogenic Bacteria Aeromonas sobria in Cultured Scortum barco and Their Antibiotic Susceptibility Test

作　　者：陈言峰[1] 冯伟强[1] 张辉华[1] 陈建酬[1] 张绮琼[1] 邹记兴[2] 付立霞[3] 

机构地区：[1]佛山科学技术学院生命科学学院,广东佛山528231 [2]华南农业大学动物科学学院,广东广州510642 [3]扬州大学动物科学与技术学院,江苏扬州225009

出　　处：《安徽农业科学》2015年第3期133-135,共3页Journal of Anhui Agricultural Sciences

基　　金：江苏省高校自然科学研究基金(11KJB240002);广东省教育部产学研项目(2010b090400255);广东省大学生创新创业训练计划项目(201411847059);广东省普通高校青年创新人才项目;佛山科学技术学院校级科研项目

摘　　要：[目的]分离并鉴定患病的高体革鯻(Scortum barco)的致病菌,并研究其药敏特性。[方法]从濒死的高体革鯻体内分离到2株致病菌(FS1491、FS1492),通过其生理生化特征与16S r DNA序列分析对其进行鉴定;通过人工感染试验测定2株致病菌对高体革鯻的半致死剂量(LD50);最后,通过药敏试验确定其对抗生素的敏感性。[结果]通过鉴定确定2株致病菌均为温和气单胞菌(Aeromonas sobria),此2株致病菌对高体革鯻的半致死剂量依次为2.1×105和8.5×104cfu/g。该菌对头孢噻肟、环丙沙星、诺氟沙星和左氟沙星等抗生素高度敏感。[结论]该研究可为防治温和气单胞菌引起的高体革鯻疾病提供参考。[Objective] Pathogenic bacteria from diseased Scortum barco were isolated and identified,and their antibiotic susceptibility was studied. [Method] Two pathogenic bacterial strains（ FS1491,FS1492） were isolated from moribund Scortum barco,and they were identified through physiological and biochemical characteristics test and 16 S r DNA sequence analysis. The half lethal dose（ LD50） values for the isolates to Scortum barco were determined by the artificial infection experiment. Besides,the antibiotic susceptibility of the isolates was studied. [Result]These two bacteria were identified to be A. sobria. The LD50 values for the isolates to healthy Scortum barco were 2. 1 × 10^5 and 8. 5 × 10^4 cfu / g,respectively. The result of antibiotic susceptibility test showed the isolates were highly susceptible to cefotaxime,ciprofloxacin,norfloxacin,levofloxacin and so on. [Conclusion]This study could provide references to prevent and control the disease of Scortum barco resulted from A. sobria.

关 键 词：高体革鯻 温和气单胞菌 鉴定 药敏试验 

分 类 号：S941.42[农业科学—水产养殖]