NACE快速测定萝芙木根中育亨宾的含量  

Rapid Determination of Yohimbine in Rauvolfia Verticillata by Non-aqueous Capillary Electrophoresis

作　　者：陈琴华[1] 魏英[1] 熊琳[1] 余飞[1] 朱军[1] 

机构地区：[1]湖北医药学院附属东风医院药物分析与筛选研究所,湖北十堰442008

出　　处：《湖北医药学院学报》2014年第6期579-581,共3页Journal of Hubei University of Medicine

基　　金：湖北省卫生和计划生育委员会计划项目(2013Z-B04);湖北省2011协同创新中心基金项目(编号:4);十堰市科技局项目(14Y55)

摘　　要：目的：建立非水毛细管电泳法（non-aqueous capillary electrophoresis,NACE）快速测定萝芙木中育亨宾的含量。方法：运用非水毛细管电泳方法,以小檗碱作为内标,缓冲液为40 mmol·L-1乙酸铵-2.0%醋酸-20%乙腈的甲醇液,检测波长220 nm,分离电压25 k V,柱温20℃,压力进样：50 mbar×3 s。结果：方法最低检测浓度为1.0μg·m L^-1,线性范围10-100μg·m L^-1,r=0.996,线性关系良好。平均回收率为97.8%,RSD为1.74%。结论：本法用于萝芙木中育亨宾的含量测定快速、准确且灵敏。Objective To develop a rapid method for the determination of yohimbine in Rauvolfia verticillata by nonaqueous capillary electrophoresis （NCAE）. Methods A NACE method has been employed using berberine as an internal standard. The separation conditions were 40 mmol · L^-1 ammonium acetate （methanol medium） ,2.0% glacial acetic acid and 20% acetonitrile in methanol as running buffer, UV detection was set at 220 nm,separation voltage was 25 kV,column temperature was 20 ℃ and pressure injection was 50 mbar × 3 s. Results The limits of detection was 1.0 · mL^-1. The concent ration of yohimbine showed a linear plot in the range of 10 - 100 μg · mL^-1 （ r =0.996）. The main recovery was found to be 97. 8%. The variation was 1.74%. Conclusion This method is rapid and accurate for the quantitative analysis of yohim- bine in Rauvolfia verticillata.

关 键 词：非水毛细管电泳 育亨宾 萝芙木 测定 

分 类 号：O658.9[理学—分析化学] R284[理学—化学]