在高浓度尿素的存在下采用制备型电泳体系进行聚丙烯酰胺凝胶电泳分离腐植酸成分  

Separation of Humic Acid Constituents by Polyacrylamide Gel Electrophoresis in the Presence of Concentrated Urea Using a Preparative Electrophoresis System

作　　者：S.Karim M.Aoyama 刘毓芳[3] 吴云彬 赵瑞华[3] 张彩凤[4] 

机构地区：[1]日本弘前大学农业和生命科学学院 [2]日本岩手大学农业科学联合研究院 [3]晋中学院化学化工学院,晋中0306002 [4]太原师范学院化学系,太原030006

出　　处：《腐植酸》2014年第6期28-31,共4页Humic Acid

基　　金：国家自然科学基金(项目编号:20875059);山西省高校科技开发(项目编号:20121026)资助项目

摘　　要：采用制备型电泳体系在高浓度的尿素存在下进行聚丙烯酰胺凝胶电泳分离水溶性腐植酸中的不同成分。在聚丙烯酰胺凝胶电泳中,根据水溶性腐植酸中深色成分分子大小的不同能够将它们彼此分离开来,同时还可以通过酸沉淀的方法将这些深色物质重新回收。水溶性腐植酸中大部分的荧光物质分子尺寸都很小,这使得它们可以溶于酸,并且被酸溶解的荧光物质可以通过吸附在DAX-8树脂上而重新获得。但是,水溶性腐植酸中的非荧光物质则会在电泳过程中丢失。而那些被认为是小分子的荧光物质则会通过氢键的断裂和7 M尿素的疏水作用得到分离。漫反射傅里叶变换红外光谱的测量结果表明腐植酸中不同成分的化学性质是不同的。而实验结果表明在高浓度尿素存在下聚丙烯酰胺凝胶电泳是分离和收集水溶性腐植酸中各组分的一种很有用的方法。To separate Humic Acid（HAs） into their constituents, Polyacrylamide Gel Electrophoresis（PAGE） was carried out in the presence of 7 M urea using a preparative electrophoresis system. Upon PAGE the dark-colored constituents of HAs were separated by their molecular sizes and recovered by precipitation with acid. Considerable parts of the fl uorescent constituents of HAs with the lowest molecular size were rendered soluble in acid and the acid soluble fl uorescent constituents were recovered by adsorption onto DAX-8 resin. However, the colorless constituents of HAs were lost during the electrophoresis. These constituents were considered as the low molecular sizes constituents separated due to disruption of the hydrogen bonding and hydrophobic interaction by 7 M urea. The measurement of diffuse refl ection infrared Fourier transform spectra indicated that the chemical properties of HA constituents were different from each other. The results show that preparative PAGE in the presence of concentrated urea is a useful method for fractionating and collecting the constituents of HAs.

关 键 词：荧光 腐植酸 腐殖化程度 制备电泳 聚丙烯酰胺凝胶电泳(PAGE) 尿素 

分 类 号：TQ314.1[化学工程—高聚物工业]