抗氮营养阻遏黄孢原毛平革菌pcR5305产酶条件优化  

Optimizing Enzyme-production Conditions of Phanerochaete chrysosporium pcR5305 which Can be Resistant to Nitrogen Nutritional Repression

作　　者：叶友贤 

机构地区：[1]漳州市辐射环境管理站,福建漳州363000

出　　处：《三明学院学报》2014年第6期87-92,共6页Journal of Sanming University

摘　　要：对选育的抗营养阻遏产酶黄孢原毛平革菌pc R5305菌株的产酶营养基质与环境条件进行了优化。实验结果表明pc R5305在37℃、初始p H4.5条件下,以酒石酸铵(富氮2.2g/L),葡萄糖(20g/L),Mn2+(0.2g/L),Tween 80(1g/L)为主要成分的优质培养基中培养24h后添加5.2mmol/L苯甲醇,pc R5305的产酶活性最高。上述条件下,该菌接种后静置培养6 d,Li P活力可达到2013.59U/L,Mn P活力达到353.42U/L,分别提高了23.41%和27.97%。We optimized the enzyme-production nutritional medium and environment condition of the breeding strain of Phanerochaete chrysosporium pcR5305 which can be resistant to nitrogen nutritional repression. Results show that pcR5305 had the best enzyme activity when it was grown at the following condition：the medium was mixed with ammonium tartrate 2.2g/L, glucose 20g/L, Mn^2＋0.2g/L and Tween 80 1g/L, and adding benzyl alcohol 5.2mmol/L after 24h, the culturing temperature was 37℃and the initial pH was 4.5. After 6d static cultured, the LiP activity could reached 2013.59U/L, and the MnP activity could reach 353.42U/L, increased respectively by 23.41% and 27.97% against the un-optimized cultured strains.

关 键 词：黄孢原毛平革菌 木素酶 抗营养阻遏 产酶优化 

分 类 号：Q939.9[生物学—微生物学]