微生物激发子对竹黄产竹红菌素的影响  

Effect of Polysaccharides from Bamboo Endophytes on Hypocrellin Production by Shiraia bambusicola

作　　者：杜文[1] 田维毅[1] 王平[1] 王文佳[1] 韩洁[1] 高小娇 曾茜[1] 郁建平[2] 韩燕峰[3] 梁宗琦[3] 

机构地区：[1]贵阳中医学院基础医学院,贵州贵阳550002 [2]贵州大学生化营养研究所,贵州贵阳550025 [3]贵州大学真菌资源研究所,贵州贵阳550025

出　　处：《食用菌学报》2014年第4期23-26,共4页Acta Edulis Fungi

基　　金：贵州省科学技术基金(编号:黔科合J字[2014]2039);贵阳中医学院2014博士基金;贵州省中医药管理局中医药;民族医药科学技术课题研究资助

摘　　要：用竹黄(Shiraia bambusicola)寄生的竹枝、叶、根为材料分离内生菌,再通过竹红菌素的抑菌效果和发酵试验初筛发现获得的4个内生细菌和2个内生真菌的粗多糖激发子都能一定程度提高竹黄菌丝中竹红菌素的含量,其中最有效的为TT1(Trametes sp.)栓菌属真菌的激发子。在竹黄培养液中加入50μg/mL的TT1激发子可明显提高竹黄菌丝生物量和菌丝中竹红菌素含量,与对照相比竹红菌素增产约80%。Four bacterial and two fungal endophytes were isolated from root,trunk and leaf samples of a bamboo plant infected with Shiraia bambusicola.Polysaccharides prepared from these endophytes acted as microbial elicitors,increasing hypocrellin production by S.bambusicola mycelium grown in submerged culture.Polysaccharide TT1 ,obtained from a Trametes sp,was the most effective elicitor;supplementation of S.bambusicola cultures with 50 μg/mL TT1 increased hypocrellin yields by -80% and also enhanced mycelial biomass production.

关 键 词：竹红菌素 竹黄 激发子 

分 类 号：S567.3[农业科学—中草药栽培]