铅对大鼠海马一氧化氮合酶和一氧化氮的影响  

Effects of Lead Exposure on NOS and NO in Rats Hippocampus

作　　者：周蔚[1] 王世鑫[2] 魏茂提[1] 王俊虹[1] 张国辉[1] 田庚友[1] 

机构地区：[1]武警医学院军事医学教研室,天津300162 [2]武警医学院附属医院,天津300162

出　　处：《工业卫生与职业病》2002年第4期222-224,共3页Industrial Health and Occupational Diseases

基　　金：中国人民武装警察部队医学院科研基金资助(WY98-0 5 )

摘　　要：目的　探讨慢性染铅过程中 ,大鼠海马一氧化氮合酶 (NOS)、一氧化氮 (NO)的变化。方法　Wistar大鼠经饮水加 0 ,18 4 ,184 0mg/L醋酸铅 (PbAc)方法染毒。海马NOS活性测定采用NOS催化L Arg和氧分子生成NO法 ,海马NO (NO-3 +NO-2 )浓度采用硝酸还原酶法。结果　与对照组相比 ,高剂量染铅组海马NOS活性在早期 (7d)显著升高 (P <0 0 5 ) ,30d后显著低于对照组 (P <0 0 5 )。低剂量组大鼠海马NOS活性第 7天开始有增高趋势 ,但差异无显著性 ,直到 6 0d后显著低于对照组 (P <0 0 5 )。低剂量染铅组大鼠海马NO含量 90d时明显低于对照组 (P <0 0 5 )。高剂量组大鼠海马NO含量在 6 0d后显著低于对照组 (P <0 0 5 )。结论　铅可导致海马NOS活性降低 ,NO含量降低。NOS活性降低及NO含量减少 ,可能是铅对海马产生神经毒作用的机制。Objective To study effects of chronic lead exposure on nitric oxide synthase(NOS)activity and concentration level of nitric oxide(NO)in rats hippocampus. Methods Wistar rats were exposed to lead by drinking 0,18 4,and 184 mg/L ,lead acetate solution respectively. NOS activity in hippocampus was determined by NOS catalyzing L Arg into NO.NO level was measured by means of nitric acid reductase. Results Compared with the control group,hippocampus NOS activity in high dose group increased significantly at the time of 7 d (P<0 05) ,but decreased after 30 days of exposure (P<0 05) .As to low dose group,it is not until after 60 days did NOS activity become lower (P<0 05) .Hippocampus NO level lowered significantly after 90 d for low dose group and after 60 d for high dose group than those of the control group (P<0 05) .Conclusions Decreased NOS activity and concentration level of NO in rats hippocampus after lead exposure might be related to the mechanism of neural toxicity of hippocampus.

关 键 词：铅染毒 海马 一氧化氮合酶 一氧化氮 

分 类 号：R135.1[医药卫生—劳动卫生]