Simultaneous determination of four triterpenoid saponins and two liganoids in the roots of Ilex pubescens by RP-HPLC-DAD  

RP-HPLC-DAD法同时测定毛冬青根中4种三萜皂苷和2种木脂素的含量(英文)

作　　者：周渊[1] 李宁[1] 张加余[2] 姜勇[1] 屠鹏飞[1,3] 

机构地区：[1]北京大学医学部 药学院 天然药物及仿生药物国家重点实验室,北京100191 [2]北京中医药大学 科研实验中心,北京100029 [3]北京中医药大学 中医药现代研究中心,北京100029

出　　处：《Journal of Chinese Pharmaceutical Sciences》2014年第12期866-872,共7页中国药学（英文版）

基　　金：Research Fund for the Doctoral Program of Higher Education of China(Grant No.20110001130003);the National Key Technology R&D Program "New Drug Innovation"of China(Grant No.2012ZX09301002-002-002 and 2012ZX09304-005)

摘　　要：A reversed-phase high performance liquid chromatography （RP-HPLC） method with diode array detector （DAD） has been established to simultaneously determine six bioactive compounds in the roots ofllex pubescens, namely tortoside A, （＋）-syringaresinol, ilexsaponin B3, ilexsaponin A1, ilexsaponin B1, ilexgenin A. The RP-HPLC assay was performed on a reversed-phase C18 column with a gradient elution. The mobile phase consisted of acetonitrile and water containing 0.1% （v/v） phosphoric acid. The flow rate was 1.0 mL/min and the detection wavelength was set at 210 nm. The six marker constituents were separated well with good linearity （r2〉0.9996）, precision, stability and repeatability. The overall recoveries were in the range of 99.00%-104.52%. Cluster analysis was employed to analyze 15 batches of samples. The result indicated this method provide an efficient way to perform quality control as well as a scientific rationale for the Geo-authentication of I. pubescens.建立了反相高效液相色谱-二极管阵列检测法同时测定毛冬青根中的6种活性成分(tortoside A,(+)-丁香脂素,毛冬青皂苷B3,毛冬青皂苷A1,毛冬青皂苷B1,毛冬青素A)的含量。RP-HPLC分离用反相C18分析柱,流动相以乙腈-0.1%磷酸水体系梯度洗脱。流速1.0 mL/min,检测波长为210 nm。6个指标成分达到基线分离,标准曲线具有良好的线性(r2>0.9996),精密度、稳定性和重复性符合分析方法学要求。加样回收率范围为99.00%–104.52%。最后对15个批次的毛冬青药材含量测定结果进行了聚类分析。结果表明该方法不仅可以对毛冬青药材进行质量控制,而且也为毛冬青药材的道地性分析提供了科学依据。

关 键 词：RP-HPLC-DAD AQUIFOLIACEAE llexpubescens Triterpenoid saponin Liganoid Cluster analysis 

分 类 号：R284[医药卫生—中药学]