锰接触工人神经行为改变与XRCC1基因C26304T和G27466A位点多态性的关系  

Relationship between neurobehavioral changes of Mn exposed workers and C26304T and G27466A in XRCC1 Gene

作　　者：姜慧[1,2] 李宏彬[3] 樊婷[4] 申旭波[2] 贾飞飞[5] 邹焰[2] 

机构地区：[1]中国疾病预防控制中心,北京102206 [2]遵义医学院公共卫生学院,贵州遵义563099 [3]新乡医学院公共卫生学院,河南新乡453000 [4]山西省疾病预防控制中心,山西太原030012 [5]中国石油大学(华东)校医院,山东东营257061

出　　处：《工业卫生与职业病》2015年第1期11-15,共5页Industrial Health and Occupational Diseases

基　　金：贵州省科技厅基金资助项目(黔科合SY[2009]3067)

摘　　要：目的探讨职业性锰接触工人DAN损伤修复基因X射线修复交叉互补基因1(XRCC1)C26304T位点和G27466A位点的多态性与神经行为改变的关系。方法选取工龄为≥0.5a的职业性锰接触冶炼工人200名和劳动强度相似不接触锰的工人94名,按照累积暴露指数将调查对象分为高、低接触组和对照组。利用垂直沟槽稳定试验和插板试验进行神经行为改变的检测;提取血白细胞DNA,利用PCR扩增限制性酶切法(PCR-RFLP)检测XRCC1C26304T位点和G27466A位点的单核苷酸多态性(SNPs)。结果高、低接触组中携带C26304T位点变异基因型TT的工人在垂直沟槽稳定试验的划痕长度、集合试验的得分均低于携带野生基因型CC和杂合基因型CT的工人,碰撞次数高于野生基因型和杂合基因型;对照组基因型之间差异无统计学意义(P>0.05)。高、低接触组中携带G27466A位点变异基因型AA的工人在垂直沟槽稳定试验的划痕长度、集合试验的得分均低于携带野生基因型GG和杂合基因型GA的工人,碰撞次数高于野生基因型和杂合基因型;对照组基因型之间差异无统计学意义(P>0.05)。结论携带XRCC1C26304T和G27466A位点变异基因型的工人是锰损伤的易感者;XRCC1基因位点C26304T和G27466A突变导致的多态性有可能作为检测锰致神经行为改变以及锰中毒风险评估的可用指标。Objective To study the relationship between neurobehavioral changes and XRCC1 gene polymorphism in manganese exposed workers. Methods 200 Mn exposed workers working over half year and 94 Mn non--exposed workers with similar work intensity were selected and divided into high exposed group, low exposed group and control group according to cumulated exposure index （CEI）. Each worker was examined by neurobehavioral detective equipment to test their neurobehavioral changes. The genetic DNA was extracted from every worker; the genotypes of C26304T and G27466A were detected by polymerase chain reaction restrained fragment length polymorphism technique （PCR-RFLP）. Results The genotype of CC, CT, TT was significantly different in three neurotoxic behavior detection tests in high-group and low-group（P 〈0.05） ; The people with TT genotype were lower than people whose genetype were CC and CT in the length of drawing and the score of assemble test; The people with TT genotype were higher than people whose genetype were CC and CT in the number of collision; there was no statistically significant difference between exposed group and control group（P〉0.05）. The genotype of GG, GA, AA was significantly different in three neurotoxic behavior detection tests in both high-group and low-group（P〈0.05）; The workers with AA genotype were lower than workers whose genetype were GG and GA in the length of drawing and the score of assemble test; the subjects with AA genotype were higher than subjects whose genetype were GG and GA inthe number of collision~ there was no statistically significant difference between exposed group and control group （ P ~ 0.05 ）. Conclusions XRCC1 C26304Tpolymorphism loci manganese exposure might affect neurobehavioral contacts; XRCC1 C26304T SNPs might be used as an indicator to detect the manganese--induced neurobehavioral changes, and also as the risk assessment in manganese poisoning.

关 键 词：锰 XRCC1基因 基因多态性 神经行为改变 

分 类 号：R135.1[医药卫生—劳动卫生]