原花青素对硫酸镍诱导大鼠生殖损伤的拮抗作用  

The protective effects of procyanidin on reproductive damage induced by nickel sulfate of male rat

作　　者：苏莉[1] 邓渊韬[2] 张瑞[1] 李成云[1] 李晋[1] 

机构地区：[1]兰州大学公共卫生学院卫生毒理学研究所,甘肃兰州730000 [2]甘肃省肿瘤医院,甘肃兰州730050

出　　处：《工业卫生与职业病》2015年第1期16-18,22,共4页Industrial Health and Occupational Diseases

基　　金：国家自然科学基金项目青年基金(8140273);兰州大学中央高校基本科研业务费专项资金(Lzujbky-2012-158)

摘　　要：目的探讨葡萄籽原花青素对镍诱导雄性大鼠生殖损伤的保护作用及机制。方法将雄性大鼠随机分为对照组、硫酸镍组和原花青素低、中、高剂量干预组。除对照组外,实验组大鼠每天均腹腔注射硫酸镍2.5mg/kg,干预组同时每天分别灌胃给予原花青素50、100和200mg/kg,连续30d。分离检测附睾和睾丸样本,检测精子活率和密度、活性氧自由基(ROS)荧光强度及干细胞生长因子受体(c-kit)蛋白表达水平。结果与对照组比较,硫酸镍组大鼠精子活率及密度明显降低,睾丸细胞ROS荧光强度上升及ckit表达上调(P<0.05)。与硫酸镍组比较,原花青素各剂量干预组大鼠精子密度均明显升高、ROS荧光强度明显降低(P<0.05),精子活率则呈上升趋势(P>0.05);原花青素中、高剂量干预组c-kit表达下调(P<0.05)。结论原花青素通过下调c-kit表达和清除ROS,拮抗硫酸镍诱导的雄性生殖损伤。Objective To explore the protective effects of grape seed procyanidin extract （GSPE） against NiSO4 toxicity. Methods Wistar rats were treated with normal saline, NiSO4 alone （2.50 mg/kg/day）, and NiSO4（2.50 mg/kg/day） plus GSPE （50,100 and 200 mg/kg/day） for 30 d. Sperm motility, density of spermatozoa （ρ）, reactive oxygen species （ROS） and the expression of c-kit were investigated. Results Significant decreases in sperm motility and density of spermatozoa （ρ） and increases in ROS and e-kit expression in the NiSO4 groups were observed compared with the control group （P〈0.05）. In contrast, all GSPE groups displayed significant increase in p and decrease in ROS compared with the NiSO4 group （P〈0.05）. Moreover, c-kit expression significantly decreased in the 100 and 200 mg GSPE groups （P 〈0.05）. Conclusions GSPE offsets reproductive damage induced by nickel through scavenging ROS and down--regulating c-kit protein expression.

关 键 词：硫酸镍 原花青素 睾丸 ROS C-KIT 

分 类 号：R114[医药卫生—卫生毒理学]