以甘油为底物鼠李糖脂高产菌株的诱变选育  

Mutating rhamnolipids high-yielding strain with glycerol as substrate

作　　者：顾生辉[1] 朱莉[1] 詹晓北[1] 吴剑荣[1] 

机构地区：[1]江南大学生物工程学院教育部糖化学与糖生物技术重点实验室,江苏无锡214122

出　　处：《生物加工过程》2015年第1期54-59,共6页Chinese Journal of Bioprocess Engineering

基　　金：国家科技支撑计划(2011ABD23B00)

摘　　要：通过诱变选育,将铜绿假单胞菌(Pseudomonas aeruginosa)RG-14利用甘油发酵生产鼠李糖脂产量由13.6g/L提高到16.5 g/L。突变株经过5次连续传代培养,菌株仍维持稳定的鼠李糖脂产量,表明该菌株具有较好的遗传稳定性。利用基质辅助激光解析电离飞行时间质谱(MALDI-TOF-MS)技术分析诱变后菌株发酵甘油生产鼠李糖脂的组成,结果显示鼠李糖脂由Rha-C8-C8、Rha-C8-C10、Rha-C10-C10、Rha-C10-C12∶1、Rha-C10-C12、Rha2-C8-C10、Rha2-C10-C10、Rha2-C10-C12∶1和Rha2-C10-C12组成,其中单、双鼠李糖脂的相对丰度分别为54.8%和45.2%。当以工业粗甘油代替精甘油为底物时,该菌株鼠李糖脂产量达到14.2 g/L,表明其具有较好的应用潜力。Through mutation, rhamnolipids yield of Pseudomonas aerugirtosa RG-14 increased from 13.6 g/L to 16.5 g/L. After 5 rounds of subculturing, rhamnolipids yield of the mutant stayed stable. Subsequently, MALDI-TOF-MS was applied to analyze the composition of rhamnolipids produced by the mutant. The results showed that the rhamnolipids congener was comprised of Rha-C8-C8, Rha-C8-C10, Rha-C10-C10, Rha-C10-C12 ： 1, Rha-C10-C12, Rha2-C8-C10, Rha2-C10-C10, Rha2-C10-C12 ： 1 and Rha2-C10-C12. The relative abundance of mono-rhamnolipids and di-rhamnolipids were 54.8% and 45.2%, respectively. The mutant strain also could use crude glycerol besides refined glycerol, and the rhamnolipids yield reached 14. 2 g/L, showing application potential of this strain.

关 键 词：铜绿假单胞菌 鼠李糖脂 亚硝基胍诱变 粗甘油 

分 类 号：Q81[生物学—生物工程]