健脾益肺Ⅱ号对熏烟联合脂多糖诱导大鼠肺组织氧化/抗氧化失衡的调节及超微结构的影响  

Effects of Jianpi YifeiⅡ Formula on Regulation of Oxidant/anti-oxidant Imbalance and Ultrastructure of Lung Tissues Induced by CSE and LPS in Rats

作　　者：林琳 于旭华 许银姬 周明娟 吴蕾 吴玲霓[2] 

机构地区：[1]广州中医药大学第二附属医院,广东广州510120 [2]广州中医药大学测试中心,广东广州510405

出　　处：《南京中医药大学学报》2015年第1期39-43,共5页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：广东省中医药科学院联合科研专项(2011B032200007);广东省科技计划项目(2011B061300074);国家自然科学基金(81373566);广东省中医药局科研课题(20121205)

摘　　要：目的探讨健脾益肺Ⅱ号调节机体氧化/抗氧化平衡的作用及其对肺组织超微结构的影响,从而阐明其防治COPD的作用机理,为临床应用提供实验依据。方法将SD大鼠随机分为正常对照组、模型组、健脾益肺Ⅱ号低剂量组、健脾益肺Ⅱ号高剂量组、强的松组。以熏烟联合气管内滴入脂多糖（LPS）的方法建立大鼠模型,给予相应药物干预,观察记录大鼠一般状况;检测各组动物气道阻力、胸腔内压力及肺顺应性;电镜下观察各组肺组织超微结构的变化;测量各组动物肺组织匀浆液中SOD活性、MDA、GSH、GSH-Px含量的变化。结果一般状态观察结果显示,模型组大鼠一般状态较差,体质量较正常组增长慢,具有统计学意义（P〈0.01）,而健脾益肺Ⅱ号组大鼠体一般状态较好,体质量增长较快（P〈0.01）;肺功能检测提示,健脾益肺Ⅱ号可以降低模型大鼠气道阻力和胸腔内压力（P〈0.05-0.01）,肺顺应性无明显改变;透射电镜结果表明,模型大鼠肺泡细胞外缘不完整,微绒毛稀疏,毛细血管外缘不完整,肺泡间隔结缔组织层增厚,健脾益肺Ⅱ号可改善模型大鼠肺组织超微结构的损伤;经过健脾益肺Ⅱ号干预后,模型肺组织中的SOD活性、GSH含量升高（P〈0.01）,而MDA含量下降（P〈0.01）。结论健脾益肺Ⅱ号能纠正熏烟联合脂多糖诱导的大鼠肺组织氧化/抗氧化失衡,保护肺组织免于氧化损伤,减轻气道阻力和胸腔压力,这可能是健脾益肺Ⅱ号方治疗COPD的作用机理之一。OBJECTIVE To investigate the effects of Jianpi Yifei Ⅱ formula on regulation of oxidant/anti-oxidant imbalance and ultrastructure of lung tissues of rats, to elucidate the mechanism of its protection from chronic obstructive pulmonary dis ease（COPD）, and provide theoretical basis for clinical practice. METHODS SD rats were randomly divided into normal group, model group, low and high close groups of Jianpi Yifei Ⅱ formula and prednisone group. The COPD rat model was es- tablished by cigarette smoke exposure （CSE） and lipopolysaccharide （LPS） intra-tracheal dripping. Appropriate drug interven tion was given in Jianpi Yifei Ⅱ groups and prednisone group respectively. General conditions of rats were observed, the air- way resistance, intra-thoracic pressure and lung compliance of rats in each group were detected, and the ultrastructure of lung tissues was observed under transmission electron microscope （TEM）. The contents of SOD, MDA, GSH and GSH-Px in lung tissue homogenates were also determined. RESULTS Rats in model group displayed poor general condition with significantly slow body weight growth （P〈0.01）, compared with the normal group. However, the general conditions of rats in Jianpi Yifei Ⅱ groups were better than those in model group, with more significantly rapid body weight growth （P〈0.01）. Compared with model group, Jianpi Yifei Ⅱ groups displayed reduced airway resistance and intra-thoracic pressure （P〈0.05-0.01）, as well as no significant change in lung compliance. TEM results showed damaged ultrastructure of lung tissues in model group with incomplete outer edge of alveolar cells, sparse microvilli, incomplete capillary outer edge and the thickening of connective tissue layers in alveolar septal. Jianpi Yifei Ⅱ formula could attenuate the ultrastructure damage of lung tissues in model rats. The levels of SOD and GSH significantly increased （P〈0. 01）, while the content of MDA decreased （P〈0. 01） after Jianpi Yifei Ⅱ formula treatment. CONCLUSIO

关 键 词：健脾益肺Ⅱ号 慢性阻塞性肺病 氧化应激 超微结构 

分 类 号：R285.5[医药卫生—中药学]