三种用于蛋白类药物载体的可降解膜的比较研究  

Comparative study of three biodegradable films for protein drug carrier

作　　者：夏婷[1,2] 李书仪 孙洪范[1,2] 俞玫[1,2] 

机构地区：[1]中国医学科学院北京协和医学院生物医学工程研究所,天津300192 [2]天津市生物医学材料重点实验室

出　　处：《国际生物医学工程杂志》2014年第6期332-336,共5页International Journal of Biomedical Engineering

基　　金：科技部科研院所技术开发专项项目（2012EG150136）

摘　　要：目的 制备3种用于蛋白类药物载体的可降解膜材料,并比较其降解性能和释药性能.方法 制备了3种不同的可降解缓控释放膜,即明胶膜、壳寡糖膜和交联壳寡糖膜.利用考马斯亮蓝测定蛋白释放量,确定膜的降解率和溶胀率.同时,采用噻唑蓝（MTT）法考察膜的生物相容性.结果 交联壳寡糖膜的释放时间（168h）大于壳寡糖膜,且不同介质中的释放时间不同,降解率和溶胀率分别为60％（360h）和110.45％,而壳寡糖膜的降解率和溶胀率分别为80％（360h）和113.03％.MTT结果表明,交联壳寡糖膜具有较好的生物相容性.结论 通过对比分析,最终确定交联壳寡糖膜为最佳蛋白类药物载体.Objective To prepare three kinds of biodegradable film materials used for protein drug carrier, and compare their degradation and drug release behavior. Methods Three different biodegradable and controlled release films, gelatin, chitosan oligosaccharides and crosslinked chitosan oligosaceharides films were prepared. Protein release behavior was determined by the Bradford. At the same time, degradation rate and swelling rate were tested, and the biocompatibility of film was investigated by MTT assay. Results The release time of erosslinked chitosan oligosaccharides film was 168 h, which was longer than that of chitosan oligosaeeharides film, and different in different solution. The degradation rate and the swelling rate of crosslinked chitosan oligosaccharides was 60% （360 h） and 110.45%, respectively, while the chitosan oligosaceharides membrane was 80% （360 h） and 113.03%. The MTT assay revealed that the crosslinked chitosan oligosaccharides film had better biocompatibility. Conclusions By comparing different properties, the crosslinked chitosan oligosaccharides film is the best choice for protein drug carrier.

关 键 词：明胶 壳寡糖 壳聚糖 牛血清白蛋白 膜材料 

分 类 号：R318.08[医药卫生—生物医学工程]