基于化学指纹图谱和多指标成分含量测定的白芷质量评价  

HPLC Fingerprints Integrated with Quantitative Determination of Multi-components for Quality Control of Angelicae Dahuricae Radix

作　　者：雷云[1,2] 王威[2] 史洋[1,3] 翟华强[2] 杨洪军[1] 许海玉[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]北京中医药大学,北京100029 [3]陕西中医学院,陕西咸阳712046

出　　处：《中国实验方剂学杂志》2015年第2期36-39,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"十一五"科技支撑计划项目(2008BAI51B03);国家自然科学基金青年基金项目(81202793);中国中医科学院基本业务费自助选题项目(Z02063);中央级公益性科研院所基本科研业务费专项(ZZ070819)

摘　　要：目的:采用高效液相色谱法建立白芷中香豆素类成分的指纹图谱,并同时测定欧前胡素和异欧前胡素的含量。方法:采用Platisil DOS C18色谱柱(4.6 mm×250 mm,5μm),乙腈-水为流动相进行梯度洗脱,检测波长254 nm。共收集了10批白芷进行测定,运用国家药典委员会"中药色谱指纹图谱相似度评价软件"2004A版进行评价,并对2种成分进行含量测定。结果:建立了白芷香豆素类成分HPLC指纹图谱,标定了10个共有色谱峰。含量测定条件通过方法学验证,平均加样回收率分别为102.52%,98.23%。结论:该法所建立的白芷香豆素类成分指纹图谱特征性强、方法简便,结合欧前胡素和异欧前胡素含量测定可更好地控制其质量,对白芷的鉴定及质量控制具有指导意义和参考价值。Objective：A method of HPLC-DAD was developed to evaluate the quality of Angelicae Dahuricae Radix through multi-target ingredient determination and finger printing analysis technologies.Method：The optimal conditions of separation and detection were achieved on a Platisil DOS C18 （4.6 mm ×250 mm,5 μm） with linear gradient elution of acetonitrile-water.The detection wavelength was 254 nm.Collect 10 batches from different origins in China to assess the similarities according to similarity evaluation for ‘ chromatographic fingerprint of traditional Chinese medicine’ （2004A）.Result：In quantitative analysis,the two components showed good regression with linear ranges,and their recoveries were 102.52％ and 98.23％.Conclusion：This study indicated that the combination of quantitative and chromatographic fingerprint analysis can be readily utilized as a quality control method for Angelicae Dahuricae Radix.

关 键 词：白芷 指纹图谱 欧前胡素 异欧前胡素 

分 类 号：R284.1[医药卫生—中药学]