柴胡疏肝汤对慢性颞叶癫痫-抑郁共病模型大鼠海马中5-HT含量及IL-1β,IL-6mRNA表达的影响  

Effect of Chaihu Shugan Decoction on Content of 5-HT and Expression of IL-1β mRNA IL-6 mRNA of Hippocampus in Chronic Temporal Lobe Epilepsy-deoression Modle Rats

作　　者：任志军[1] 谢炜[1,2] 刘远征[1] 郑跃辉[1] 

机构地区：[1]南方医科大学中医药学院,广州510515 [2]南方医科大学南方医院中医科,广州510515

出　　处：《中国实验方剂学杂志》2015年第2期115-119,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81173458);国家自然科学基金面上项目(30973848);广东省自然科学基金项目(S2011020003207);高等学校博士学科点专项科研基金(201144331100);广东省科技计划项目(2013A032500004)

摘　　要：目的:观察柴胡疏肝汤对慢性颞叶癫痫-抑郁共病模型大鼠海马中5-羟色胺(5-HT)含量及白介素-1β(IL-1β),白介素-6(IL-6)mRNA表达的影响。方法:腹腔注射氯化锂-匹罗卡品制作癫痫-抑郁共病大鼠模型,将成功模型随机分为模型组,西酞普兰组,柴胡疏肝汤高、中、低剂量组。正常组10只。药物干预组分别给予西酞普兰10 mg·kg-1·d-1,柴胡疏肝汤高、中、低剂量10,5,2.5 g·kg-1·d-1,正常组与模型组对应给予等量生理盐水ig,连续4周,2次/d。通过强迫游泳(FST)和蔗糖消耗试验评估抑郁行为的变化;视频录像监测大鼠痫性发作次数;液相色谱-质谱法检测海马5-HT的含量;实时荧光定量PCR检测海马IL-1β,IL-6 mRNA的表达量。结果:与正常组比较,模型组大鼠FST累计不动时间明显延长,IL-1βmRNA表达量明显增加,糖水消耗量明显减少(P<0.01),5-HT含量减少,IL-6 mRNA表达量增加(P<0.05)。与模型组比较,药物干预4周后,西酞普兰,柴胡疏肝汤高、中剂量组大鼠痫性发作次数明显减少,FST累计不动时间明显缩短,糖水消耗量明显增加,海马中IL-1β,IL-6 mRNA的表达明显下调﹙P<0.01﹚;5-HT的含量增加﹙P<0.05﹚。西酞普兰组与柴胡疏肝汤高、中剂量组比较,各指标差异均无统计学意义。结论:柴胡疏肝汤高、中剂量具有抑制慢性颞叶癫痫-抑郁共病模型大鼠海马中IL-1β,IL-6mRNA表达,促进5-HT表达,减少大鼠痫性发作次数,改善抑郁行为的作用。Objective：To observe the effect of Chaihu Shugan decoction （CHSGD） on the content of serotonin （5-HT）,interleukin-1beta （IL-1β3）,interleukin-6 （IL-6） mRNA expression of hippocampus in chronic temporal lobe epilepsy and depression model rats.Method：The intraperitoneal injection of lithium chloride-pilocarpine was used to make epileptic-depression model,the modeling rats were randomly divided into model group,citalopram group,CHSGD-high,medium,low dose group and normal group.The drug intervention group was given citalopram 10 mg·kg^-1d^-1,CHSGD-H,M,L at dose of 10,5,2.5 g·kg^-1d^-1 orally,normal group and model group were given equal salineby continuous lavage for 4 weeks,twice each day.By forced swim test （FST） and the changes of sucrose consumption the depressive behavior was assessed,video was usec to monitor seizures,hippocampal 5-HT number was determined by liquid chromatography mass spectrometry.Hippocampal IL-1β,IL-6 mRNA expression was detected by real-time fluorescence quantitative PCR detection.Result：Compared with normal group,model group rats FST total fixed time extended obviously,the sugar water consumption decreased significantly,IL-1β mRNA expression quantity increased obviously （P 〈 0.01） ; 5-HT content reduced,IL-6 mRNA expression increased （P 〈 0.05）.Compared with model group,drug intervention after 4 weeks,citalopram,CHSGT-H,M dose group of seizures in rats reduced significantly,FST total fixed time shortened significantly,sugar water consumption increased significantly,the hippocampus IL-1β,IL-6 mRNA expression lowered significantly （P 〈 0.01）,the content of 5-HT increase （P 〈 0.05）.Citalopram group and CHSGD-H,M dose group of comparison,there was no statistical significance.Conclusion：High,medium dose of Chaihu Shugan decoction can inhibit epilepsy comorbidity of depression in hippocampus of modle rats IL-1β3,IL-6 mRNA expression,promote the expression of 5-HT,reduce the number of seizures and improve depressive behavior in rats.

关 键 词：柴胡疏肝汤 癫痫-抑郁共病 5羟色胺 白介素-1Β 白介素-6 

分 类 号：R285.5[医药卫生—中药学]