山楂叶总黄酮对脑缺血再灌注损伤大鼠神经元凋亡和c-fos,c-jun表达的影响  

Impact of Total Flavone of Hawthorn Leaf on Neuronal Apoptosis and c-fos and c-jun Expression in Rat Cerebral Ischemia-reperfusion Injury

作　　者：毛晓霞[1] 夏爱华[1] 吴晓光[2] 苗光新[1] 

机构地区：[1]河北省中药研究与开发重点实验室承德医学院,河北承德067000 [2]基础医学研究所承德医学院,河北承德067000

出　　处：《中国实验方剂学杂志》2015年第2期174-177,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河北省卫生厅重点课题(20120160);河北省高等学校科学研究计划项目(QN2014103);河北省中医药抗痴呆重点研究室资助项目;河北省重点学科资助项目

摘　　要：目的:观察山楂叶总黄酮(TFHL)对脑缺血再灌注损伤大鼠神经元凋亡及c-fos,c-jun基因表达的影响。方法:80只SD大鼠随机分为假手术组、模型组、TFHL 70,140 mg·kg-1组和银杏叶片3.5 mg·kg-1组,每组16只。四血管阻断法建立全脑缺血再灌注损伤模型,穿梭箱实验检测学习记忆能力;分光光度比色法检测血清超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量;酶联免疫吸附测定(ELISA)法检测半胱氨酸蛋白酶-3(caspase-3)的含量;免疫组化法检测c-fos及c-jun的表达。结果:与假手术组比较,模型组大鼠学习记忆能力显著降低,大鼠主动逃避反应率明显降低(P<0.01),脑组织凋亡细胞显著增多(P<0.01);c-fos与c-jun基因表达明显增强(P<0.01);与模型组比较,TFHL及银杏叶片组大鼠主动逃避反应率增高(P<0.05),SOD活性明显升高(P<0.05),MDA含量显著降低(P<0.05),凋亡细胞明显减少(P<0.05),c-fos与c-jun基因表达显著降低(P<0.05)。结论:TFHL可通过降低脑缺血再灌注损伤脑组织c-fos与c-jun基因表达、抑制神经元凋亡、改善脑缺血再灌注损伤大鼠的神经功能,提高大鼠的学习记忆能力。Objective：To observe the total flavone of hawthorn leaf （TFHL） on apoptosis of cerebral ischemia reperfusion injury in rats and c-fos,c-jun the effects of gene expression.Method：Eighty SD rats were randomly divided into sham group,model group,TFHL 70 mg ·kg-1 group,140 mg ·kg-1group and Yinxingye tablet 3.5 mg ·kg-1 group,and each group of 16.Four blood vessel blocking method to establish the whole cerebral ischemia reperfusion injury model of shuttle box test to detect the ability of learning and memory; spectrophotometric assay superoxide dismutase （SOD） activity and malondialdehyde （MDA） content; enzymelinked immunosorbent assay （ELISA） method to detect the content of caspase-3 ; immunohistochemical method to detect c-jun and the expression of c-fos.Result：Compared with sham group,model group rats learning and memory ability,active rats escape response rate significantly decreased （P ＜ 0.01）,Brain tissue apoptosis cells increased significantly （P ＜ 0.01） ; c-fos and c-jun gene expression obviously increased （P ＜ 0.01）.Compared with model group,TFHL active escape response rate and Yinxingye tablet group rats increased （P ＜ 0.05）,SOD activity was significantly increased （P ＜ 0.05）,MDA content was significantly lower （P ＜ 0.05）,apoptotic cells decreased significantly （P ＜ 0.05）,c-fos and c-jun gene expression decreased significantly （P ＜ 0.05）.Conclusion：TFHL can reducing cerebral ischemia reperfusion injury of brain tissue c-fos and c-jun gene expression,inhibition of neural cell apoptosis,improving cerebral ischemia-reperfusion injury in rats of nerve function,improve the learning and memory ability of rats.

关 键 词：山楂叶总黄酮 缺血再灌注 凋亡 C-FOS C-JUN 

分 类 号：R285.5[医药卫生—中药学]