GC法同时测定复方牙痛酊中樟脑和乙酸龙脑酯的含量  

Simultaneous determination of camphor and borneol acetate in compound Yatong tincture by GC

作　　者：汪建君[1] 李玲玲[1] 黄惠琼[1] 

机构地区：[1]厦门市药品检验所,厦门361012

出　　处：《药物分析杂志》2015年第1期129-132,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的：建立GC法同时测定复方牙痛酊中樟脑和乙酸龙脑酯的含量。方法：采用DB-WAX毛细管柱（30 m×0.25 mm×0.25μm）,氢火焰离子检测器（FID）;以萘为内标;进样口温度230℃,检测器温度250℃,程序升温（初始温度105℃,保持3min,以3℃·min^-1的速率升至140℃,再以100℃·min^-1的速率升至210℃,保持5 min）,分流进样,分流比2.0∶1,进样量1μL。结果：樟脑、乙酸龙脑酯浓度分别在1.81-27.18μg·m L^-1和5.53-82.95μg·m L^-1范围内呈良好的线性关系（r=0.999 9）,平均回收率（n=9）分别为103.9%和105.2%。结论：本方法可用于测定复方牙痛酊中樟脑和乙酸龙脑酯的含量。Objective： To establish a GC method for simultaneous determination of contents of camphor and borneol acetate in compound Yatong tincture. Methods： A DB-WAX capillary column（ 30 m × 0. 250 mm × 0. 25 μm）and a FID were adopted. Naphthalene was used as an internal standard substance. The injector temperature was230 ℃ and the detector temperature was 250 ℃. The initial temperature was 105 ℃,maintained for 3 min,rose to140 ℃ with a rate of 3 ℃ ·min^-1,then rose to 210 ℃ with a rate of 100 ℃·min^-1,maintained for 5 minutes.The split ratio for split stream sampling was 2. 0 ∶ 1. The injection volume was 1 μL. Results： The linear ranges of camphor and borneol acetate were 1. 81-27. 18 μg·m L^-1（ r =0. 999 9） and 5. 53-82. 95 μg·m L^-1（ r =0. 999 9）,respectively. Their average recovery rates（ n = 9） were 103. 9 % and 105. 2 %,respectively. Conclusion：The developed method is suitable for the determination of camphor and borneol acetate in compound Yatong tincture.

关 键 词：复方牙痛酊 樟脑 乙酸龙脑酯 樟木 宽叶缬草 苗药 气相色谱法 含量测定 

分 类 号：R917[医药卫生—药物分析学]