HPLC法同时测定桑枝中桑皮苷A和桑皮黄素的含量  

Simultaneous Content Determination of Mulberroside A and Mulberrin in Ramulus mori by HPLC

作　　者：陈倩[1,2] 孙登阳 邓霖芳 刘江云[1] 郝丽莉[1] 

机构地区：[1]苏州大学药学院,江苏苏州215123 [2]常州技师学院,江苏常州213000

出　　处：《中国药房》2015年第3期364-366,共3页China Pharmacy

基　　金：国家自然科学基金资助项目(No.81274190)

摘　　要：目的：建立同时测定桑枝中桑皮苷A和桑皮黄素含量的方法。方法：采用高效液相色谱法。色谱柱为Cosmosil C18-AR-Ⅱ,流动相为乙腈-1%乙酸水溶液（梯度洗脱）,检测波长为303 nm,柱温为30℃,流速为1.0 ml/min,进样量为10~20μl。结果：桑皮苷A和桑皮黄素的质量浓度分别在0.101 5~2.029、0.020 42~0.408 4 mg/ml范围内与各自峰面积积分值呈良好线性关系（r=0.999 8、0.999 7）;精密度试验的RSD〈2%,重复性、稳定性试验的RSD〈3%;平均加样回收率分别为98.2%、97.5%,RSD分别为1.39%、1.29%（n=6）。在早春采集的样品中桑皮苷A和桑皮黄素的含量较高。结论：该方法操作简便、结果准确,可为桑枝的合理采收和开发利用提供参考。OBJECTIVE： To establish a method for the content determination of mulberroside A and mulberrin in Ramulus mori. METHODS： HPLC method was adopted. The determination was performed on Cosmosil C18-AR- Ⅱcolumn with mobile phase consisted of cetonitrile-1% acetic acid aqueous solution （gradient elution） at the flow rate of 1.0 ml/min. The sample size was 10-20 μl. The detection wavelength was set at 303 nm, and column temperature was maintained at 30 ℃. RESULTS： The linear range were 0.101 5-2.029 mg/ml for mulberroside A （r=0.999 8） and 0.020 42-0.408 4 mg/ml for mulberrin （r=0.999 7）. RSD of precision test was lower than 2%, and RSDs of reproducibility and stability test were lower than 3%. Average recovery rates were 98.2% （RSD=1.39%, n=6） and 97.5% （1.29%, n=6）, respectively. The contents of mulberroside A and malberrin were higher in samples collected in early spring. CONCLUSIONS： The method developed is simple and accurate, and can provide reference for the utilization and development of R. mori.

关 键 词：桑枝 桑皮苷A 桑皮黄素 含量测定 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]