HPLC法测定不同生长年限的葛根不同部位中葛根素的含量  

Content Determination of Puerarin in Different Parts of Pueraria lobata with Different Growth Periods by HPLC

作　　者：刘计权[1] 宋强[1] 杨文珍[1] 任剑锋[1] 宋晓春[1] 

机构地区：[1]山西中医学院中药学院,太原030024

出　　处：《中国药房》2015年第3期366-368,共3页China Pharmacy

基　　金：国家科技支撑计划项目(No.2011BAI07B05);山西省卫生厅科技攻关计划项目(No.20100223);山西省中药材;中药饮片地方标准研究项目(No.2011003B)

摘　　要：目的：建立测定不同生长年限的葛根不同部位中葛根素含量的方法。方法：采用高相液相色谱法。色谱柱为Dikma Diamonsil C18,流动相为甲醇-水（25∶75,V/V）,流速为1.0 ml/min,柱温为室温,检测波长为250 nm。结果：葛根素的进样量在0.18~1.98μg范围内与其峰面积积分值呈良好线性关系（r=0.999 8）;精密度、稳定性试验的RSD〈2%,重复性试验的RSD〈3%;平均加样回收率为97.01%,RSD=1.71%（n=6）。葛根木质部与韧皮部的葛根素含量相近,茎部最少,且三年生葛根的葛根素含量最高。结论：葛根素含量与生长年限及不同部位有关。该方法操作简单、结果可靠,适用于葛根药材的质量控制。OBJECTIVE ： To establish the method for the content determination of puerarin in different parts of Pueraria lobata with different growth periods. METHODS： The sample was determined by HPLC. The determination was performed on Dikma Diamonsil C18 column with mobile phase consisted of methanol-water （25： 75, V/V） at the flow rate of 1.0 ml/min. The column temperature was ambient temperature and the detection wavelength was 250 nm. RESULTS： The linear range of puerarin was 0.18-1.98 pg with an average recovery（r=0.999 8） of 97.01% （RSD=l.71%, n=6）. RSDs of precision, stability tests were lower than 2%, and that of reproducibility test was lower than 3 %. The content of puerarin in xylem of P. lobata was similar to that in phloem of P. lobata. The content of puerarin was the highest in triennial P. lobata. CONCLUSIONS： The content of puerarin is affected by different growth periods and different parts. The method is simple and reliable, and can provide reference for standard cultivation and utilization of E lobata.

关 键 词：葛根 葛根素 高效液相色谱法 年限 木质部 韧皮部 茎部 

分 类 号：R927.2[医药卫生—药学]