电聚合茜素黄R构建辣根过氧化物酶传感器的研究  

The study on the fabrication of a horseradish peroxidase biosensor based on the electropolymerization of alizarin yellow R

作　　者：杨绍明[1] 尚培玲 孙清[1] 李瑞琴[1] 陈延胜[1] 丁素游 

机构地区：[1]华东交通大学理学院化学化工系,南昌330013

出　　处：《分析试验室》2015年第1期6-11,共6页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金项目(21465012;21065004)资助

摘　　要：利用电聚合茜素黄R（AYR）的方法，将辣根过氧化物酶（HRP）和细胞色素c（CytC）固载于通过一步法电沉积的碳纳米管一金纳米粒子（MWCNTs-AuNPs）复合纳米材料修饰电极表面，构筑PAYR-HRP-Cyt c／MWCNTs-AuNPs修饰电极，并利用HRP对H2O2的直接电化学催化行为对H2O2进行检测。采用扫描电镜对MWCNTs-AuNPs和PAYR-HRP-CytC的表面形貌进行表征。利用电化学阻抗对修饰电极的构筑过程进行了监测。采用循环伏安法和计时电流法对修饰电极的电化学行为进行了研究。探讨了pH和电位对该修饰电极测定H2O2的性能的影响。该传感器对H2O2在5．0×10^-7～3．14×10^-3mot／L范围内呈良好的线性响应，相关系数为0．9997，灵敏度为0．50A·L／mol，检出限（S/N=3）为9．6×10^-8mol／L。Through the electropolymerization of alizarin yellow R, horseradish peroxidase （HRP） and cytochrome c （Cyt c ） were immobilized on the surface of an electrode modified with nanocomposites of muhiwalled carbon nanotubes-gold nanoparticles （MWCNTs-AuNPs） for the fabrication of a PAYR-HRP-Cyt c/ MWCNTs-AuNPs modified electrode, in which MWCNTs-AuNPs was synthesized by one-step electrodeposition method. Hydrogen peroxide was detected by using the direct electrochemical catalysis with HRP. SEM was used to characterize the morphology of MWCNTs-AuNPs and PAYR-HRP-Cyt c/MWCNTs-AuNPs. Electrochemical impedance spectroscopy was used to monitor the fabrication process of the modified electrode. The effects of pH and work potential on the response of modified electrode were investigated. The biosensor shows a good linear range of 5.0 × 10^-7 - 3.14 × 10^-3 mol/L mol/L for H202, R = 0. 9997, with a detection limit of 9. 6 ×10^-8mol/L mol/L and sensitivity of 0. 5A· L/mol.

关 键 词：辣根过氧化物酶 聚茜素黄R 过氧化氢 传感器 

分 类 号：O657.1[理学—分析化学]