5-BrSAF-盐酸头孢他美酯荧光体系的研究及分析应用  

Study on the fluorescence system of 5-BrS AF-cefetamet pivoxil hydrochloride and its analytical application

作　　者：马红燕[1] 高春燕[1] 任岗[1] 李云云[1] 

机构地区：[1]延安大学化学与化工学院分析化学研究所,延安716000

出　　处：《分析试验室》2015年第1期40-43,共4页Chinese Journal of Analysis Laboratory

基　　金：陕西省自然科学基础研究计划项目(2014JM2056)资助

摘　　要：在pH7．40的磷酸盐（PBS）缓冲溶液中，盐酸头孢他美酯（CPH）可使5一溴水杨基荧光酮（5-BrSAF）的内源性荧光显著猝灭，据此建立了荧光猝灭法测定CPH的新方法。CPH的浓度在8．0×10^-10～1．8×10^-8mol／L范围内与体系的荧光猝灭值△F呈良好的线性关系，方法检出限为3．8×10^-10mol／L，相对标准偏差为1．8％（n=5，C=8．0×10^-9mol/L）。方法用于样品中CPH的测定，回收率为98．0％～103．5％。对体系的猝灭机理进行了研究，通过测定荧光寿命、探讨温度对猝灭常数的影响以及紫外吸收光谱的变化，确定CPH与5-BrSAF之间的猝灭过程为静态猝灭。In pH 7.40 phosphate buffer solution （PBS）, a novel fluorescence quenching method for the determination of cefetamet pivoxil hydrochloride （CPH） with 5-bromo-salicyl-fluorone （5-BrSAF） as a fluorescent reagent was established firstly. When the concentration of CPH in the range of 8.0 × 10^ -10 ～1.8 × 10^ -8 mol/L and the value of △ F of the fluorescence quenching system showed a good linear relationship with the concentration of CPH, the detection limit was 3.8 × 10^-10 mol/L, the relative standard deviation was 1.8% （n =5, C =8.0 × 10^-9 mol/L）. The method has been used for the determination of CPH in samples, and the recoveries were 98.0% ～ 103.5%. At the same time, the interaction mechanism of CPH and 5-BrSAF was investigated by fluorescence lifetime, the temperature effect on the quenching constants and UV absorption spectrometry, and the results showed that the interaction mechanism between CPH and 5-BrSAF was a static quenching process.

关 键 词：荧光猝灭法 5-溴水杨基荧光酮 盐酸头孢他美酯 作用机理 

分 类 号：O657.3[理学—分析化学]