布鲁氏菌LPS对小鼠巨噬细胞中NLRP3炎症小体转录水平的影响  

Effect of Brucella LPS on NLRP3-inflammasome Transcriptional Level of Mouse Macrophages

作　　者：袁莎[1] 陈鹏博[1] 陈创夫[1] 

机构地区：[1]石河子大学动物科技学院,石河子832003

出　　处：《中国畜牧兽医》2015年第1期9-16,共8页China Animal Husbandry & Veterinary Medicine

基　　金：国家重点基础研究发展计划(973计划)项目(2010CB530203)

摘　　要：为研究布鲁氏菌LPS对巨噬细胞中NLRP3炎症小体的影响,本试验提取布鲁氏菌2308、RB51和△WbkA的LPS,以不同浓度与小鼠巨噬细胞相互作用,荧光定量PCR检测其对NLRP3、ASC、Caspase1、IL1-β、IL18转录水平的影响。结果显示RB51LPS和△WbkA LPS上调NLRP3炎症小体相关基因的转录水平,且呈浓度依赖性,而浓度对2308LPS调节NLRP3炎症小体相关基因转录的作用不大;且同一浓度下,RB51LPS和2308LPS比△WbkA LPS更好的调节Caspase1、IL1-β、IL18的转录水平。In order to find out the impact of Brucella LPS on macrophages NLRP3-inflammasome,the mouse macrophages were infected by the different concentrations of LPS which extracted from Brucella2308,RB51and△WbkA to explore the changes of NLRP3-inflammasome related genes,ASC,caspase1,IL1-β,IL18 at transcription levels using Real-time PCR.The results showed RB51 LPS and △WbkA LPS regulating NLRP3-inflammasome related genes transcription relied on concentrations,and concentrations of 2308 LPS had little effect on NLRP3-inflammasome related genes transcription;And under the same concentration,RB51 LPS and 2308 LPS better regulated Caspase1,IL1-β,IL18 transcriptions than △WbkA LPS.

关 键 词：布鲁氏菌LPS 小鼠巨噬细胞 NLRP3炎症小体 

分 类 号：Q78[生物学—分子生物学]