内脂素对脂多糖刺激的大鼠肝脏组织结构和髓过氧化物酶的影响  

Effect of Visfatin on Morphological Structure and MPO in Liver of LPS-induced Rats

作　　者：杨智[1] 高敏 肖珂[1] 王为[1] 宋卉[1] 彭克美[1] 

机构地区：[1]华中农业大学动物医学院,武汉430070 [2]招远市畜牧兽医总站,招远265400

出　　处：《中国畜牧兽医》2015年第1期118-123,共6页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(31101776)

摘　　要：本试验旨在观察内脂素(visfatin)对脂多糖(LPS)刺激的大鼠肝脏组织结构和髓过氧化物酶(myeloperoxidase,MPO)的影响。试验选用24只8周龄Wistar大鼠,随机分成4组:生理盐水组、visfatin组、LPS组、visfatin+LPS组。其中生理盐水组和LPS组每天注射生理盐水,visfatin组和visfatin+LPS组每天注射visfatin,1周后,LPS组和visfatin+LPS组的Wistar大鼠注射一定量的LPS,6h后剖杀全部Wistar大鼠。采用HE和免疫组化染色观察各组Wistar大鼠肝脏的组织结构和MPO,结果发现,与生理盐水组相比,visfatin组的巨噬细胞增多,MPO表达水平显著降低(P<0.05);与LPS组相比,visfatin+LPS组的Wistar大鼠肝细胞形态变得模糊不清,炎性细胞的浸润更加明显,MPO的水平表达也显著降低(P<0.05)。结果表明,visfatin可增加肝脏中巨噬细胞的数量且对肝脏中的MPO表达水平也有显著的影响。The present study was aimed to investigate the effects of visfatin on the morphological structure and MPO in liver of LPS-induced rats.24 8-week-old Wistar rats were randomly divided into 4groups including saline group,visfatin group,LPS group and visfatin＋LPS group.Wistar rats in saline group and LPS group were both received NaCl solution injection each day,Wistar rats in visfatin group and visfatin＋LPS group were both received the injection of visfatin each day.One week later,LPS was injected into Wistar rats in LPS group and visfatin＋LPS group.All the Wistar rats were sacrificed 6hafter the injection of LPS.HE and immunohistochemical methods were used to observe the morphological structure and MPO in liver of each group.The present study showed that the macrophage increased and the expression level of MPO reduced significantly in visfatin group than saline group（P〈0.05）.Compared with LPS group,in vifatin＋LPS group,morphological of hepatocytes became disorder,the infiltration of inflammatory cells became more clear,the expression level of MPO reduced significantly（P〈0.05）.The results suggested that visfatin could increase the number of macrophage and had a remarkable effect on MPO level in liver.

关 键 词：内脂素 LPS WISTAR大鼠 肝脏 MPO 

分 类 号：Q593.1[生物学—生物化学]