细毛羊皮肤组织中毛囊蛋白质2-DE图谱的建立与初步分析  

Establishment and Preliminary Studies on the Skin Tissue Hair Follicles Proteins of 2-DE Map Building in Fine-wool Sheep

作　　者：杨洁[1] 付雪峰[2] 黄锡霞[1] 徐新明[2] 石刚[2] 詹振宏[2] 张恩平[3] 田可川[2] 

机构地区：[1]新疆农业大学,乌鲁木齐830052 [2]新疆畜牧科学院,乌鲁木齐830000 [3]西北农林科技大学,杨凌712100

出　　处：《中国畜牧兽医》2015年第1期124-130,共7页China Animal Husbandry & Veterinary Medicine

基　　金：国家绒毛用羊产业技术体系建设专项(CARS-40);国家农业杰出人才培养计划项目;新疆维吾尔自治区研究生科研创新项目(XJGRI2013110);家养动物种质资源平台项目

摘　　要：本研究以不同纤维直径的细毛羊皮肤组织中的毛囊作为试验材料,利用不同的提取方法、不同pH范围的IPG胶条以及不同的上样量,探索适用于细毛羊皮肤组织的双向电泳体系,建立细毛羊皮肤组织毛囊的2-DE凝胶图谱。凝胶图谱用ImageMaster 2DPlatinum软件自动检测蛋白点比较不同纤维直径的细毛羊差异蛋白。结果显示,TCA/丙酮抽提法最适合细毛羊毛囊组织总蛋白质的提取。上样量为100μL,选择18cm、pH 4~7的线性IPG胶条,得到质量较好的双向凝胶电泳图谱,有35个蛋白差异点,为后续优质细毛羊的蛋白质组学研究工作奠定基础。In use of hair follicles in skin tissue of fine-wool sheep with different fiber diameters as experimental material,and different extraction methods,IPG strips with different pH and different sample volume as experimental methods,this paper was aimed to explore two-dimensional electrophoresis system applied to skin tissue of the fine-wool sheep and establish 2-DE gel map of skin tissue in fine-wool sheep.Gel maps using ImageMaster 2DPlatinum software automatically detected protein points to compare different protein fiber diameter of fine-wool sheep differences.The results showed that the TCA/acetone extraction method was most suited to fine-wool sheep hair follicle tissue extraction of total protein.Choose to 18 cm,pH 4to 7linear IPG strip,we got better quality two-dimensional gel electrophoresis,got 35 protein variations among the different cantons for the follow-up work fine-wool sheep with high quality of the study of proteomics.

关 键 词：细毛羊 双向电泳 毛囊 蛋白质组学 

分 类 号：TQ93[化学工程]