贵州半细毛羊ADAMTS1基因部分外显子多态性研究  

Study on SNP for Part of ADAMTS1 Exons in Guizhou Semifine-wool Sheep

作　　者：张琼娣 宋德荣 刘若余[2] 彭华 周大荣 吴蕊汝 吴萍 

机构地区：[1]毕节市畜牧兽医科学研究所,毕节551700 [2]贵州大学动物科学学院,贵阳550025

出　　处：《中国畜牧兽医》2015年第1期172-178,共7页China Animal Husbandry & Veterinary Medicine

基　　金：国家现代农业产业技术体系专项资金(CARS-40-30;CARS-40-46);贵州省农业动植物育种专项(黔农育专字[2011]016号);毕节地区科技计划项目(毕科合字[2010]05号);贵州省科技厅;毕节地区行署;中科院昆明分院科技合作项目(省地院合2010-05);毕节试验区肉羊产业发展研究人才团队(毕人领办通[2013]7号)

摘　　要：为探索ADAMTS1基因在绵羊繁殖中的作用,本研究以贵州半细毛羊为试验对象构建DNA池,分别设计引物扩增第1、2、5-7和9外显子,研究ADAMTS1基因的遗传多态性。结果显示,ADAMTS1基因第6外显子有1个SNP(A255C),第9外显子有4个SNPs(A127G、G243T、G303T、A401G)。生物信息学分析表明,SNPs位点对ADAMTS1基因RNA二级结构和蛋白结构均有一定影响。ADAMTS1基因可能影响贵州半细毛羊的繁殖性状。In order to explore the role of ADAMTS1 gene in sheep breeding,in this study,Guizhou semifine-wool sheep were used to construct DNA pools.The complete sequences of the 1th,2th,5-7th and 9th exons and part of introns in ADAMTS1 were obtained by designing specific primers to study the genetic polymorphism of ADAMTS1 gene.The results showed that one SNP（A255C）was found in exon 6,and four（A127G,G243 T,G303T,A401G）SNPs were found in exon 9.Bioinformatics analysis showed that the polymorphisms led to the changes of RNA secondary structure and protein structure.ADAMTS1 gene might affect reproductive performance of Guizhou semifine-wool sheep.

关 键 词：贵州半细毛羊 ADAMTS1基因 核苷酸 遗传多态性 

分 类 号：S813.3[农业科学—畜牧学]