扭角羚肺炎克雷伯氏菌的分离鉴定  

Isolation and Identification of K.pneumoniaein Takin

作　　者：郝中香[1] 廖红[1] 刘丹[1] 郭玲[1] 杨绍林[1] 文彩芳[1] 张曼丽[1] 罗露[1] 郗立新 杨艳[2] 谌利民[2] 颜其贵[1,3] 

机构地区：[1]四川农业大学动物医学院,雅安625014 [2]唐家河国家级自然保护区,广元628100 [3]四川农业大学动物疫病与人类健康四川省重点实验室,雅安625014

出　　处：《中国畜牧兽医》2015年第1期203-208,共6页China Animal Husbandry & Veterinary Medicine

基　　金："国家林业局陆生野生动物疫源疾病监测"项目

摘　　要：本试验旨在报告四川省野生扭角羚自然生存过程中致病性细菌的检测及生物学特征。试验对发病死亡扭角羚病变组织进行细菌学检查及分离,对分别从肝脏、脾脏和肺脏分离得到的4株优势菌进行形态特征、生化特性和16SrDNA分子鉴定,判定为肺炎克雷伯氏菌。这4株菌对供试小白鼠均有致病性。测其LD50,分别为2.9×107、2.9×107、4.5×107和1.1×108 CFU/只。4株分离菌对供试的先锋霉素等敏感,对阿米卡星等中度敏感,对红霉素等耐药。推测这4株肺炎克雷伯氏菌是导致扭角羚死亡的主要病原菌。In order to report the detection and main biological characteristics of pathogenic bacteria isolated from wild takin in the process of the natural survival,four strains of the predominant bacteria from pathologic tissues of dead takin’s livers,spleens and lungs were examined and isolated.The detections of biochemical characteristics and 16 SrDNA gene were conducted.The strains were isolated and identified as K.pneumoniae.The four isolates had pathogenicity to mice.LD50 of the four isolates were 2.9×107,2.9×107,4.5×107 and 1.1×108 CFU,respectively.The antibiotic sensitivity test revealed that the strains were sensitive to cephalothin and so on,moderate sensitive to amikacin and so on,and resistant to erythrocin and so on.The four strains of K.pneumoniae were the primary bacterial pathogen leading to the death of takin.

关 键 词：扭角羚 肺炎克雷伯氏菌 分离鉴定 LD50 

分 类 号：S852.61[农业科学—基础兽医学]