MDA5在先天性免疫抗病毒作用中的研究进展  

Research Progress on Antiviral Effect of MDA5 in Innate Immunity

作　　者：刘欢欢[1] 谢丽君[1] 邵志勇[1] 高洪[1] 严玉霖[1] 

机构地区：[1]云南农业大学动物科学技术学院,昆明650201

出　　处：《中国畜牧兽医》2015年第1期230-233,共4页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(31160496;31360599);云南省高校动物性食品安全与人兽共患病科技创新团队项目

摘　　要：黑色素瘤分化相关基因5(melanoma differentiation-associated gene-5,MDA5)是胞浆内核酸受体,与病原相关分子模式(pathogen-associated molecular patterns,PAMPs)相结合,特异性地识别较长的双链RNA,功能与视黄酸诱导表达基因Ⅰ(retinoic acid-inducude geneⅠ,RIG-Ⅰ)相似,通过自身级联激活和招募结构域(CARD)与接头蛋白CARD同源相互作用之后,与接头蛋白线粒体连接蛋白(MAVS)(也叫VISA、Cardif或IPS-1)结合,相互作用后会导致RIG-Ⅰ样受体(RLR)在内膜上重新定位,一边招募来TRAF2/TRAF6活化IKK激酶复合物,从而激活转录因子NF-κB;另一边招募来TRAF3和TBK1,从而促进IRF3的磷酸化激活,活化后的转录因子NF-κB及IRF会进入细胞核,共同协作促进Ⅰ型干扰素基因的表达。Melanoma differentiation-associated gene-5（MDA5）was a nucleic acid receptor in the cytoplasm,in combination with pathogen-associated molecular patterns（PAMPs）,which specifically recognized a long double-stranded RNA,function was similar with retinoic acid-inducude geneⅠ（RIG-Ⅰ）,after CARD-CARD interaction,it was combinated with the adaptor protein MAVS（also called VISA,Cardif or IPS-1）,the interaction might result in the reposition of RLRs in intima,one side it recruited TRAF2/TRAF6 to activate IKK kinase complexes,thereby activating the transcription factor NF-κB;on the other side it recruited TRAF3 and TBK1,thereby promoting phosphorylated activation of IRF3,after activations of the transcription factor NF-κB and IRF,they would enter the nucleus,and work together to promote the expression of typeⅠ interferon gene.

关 键 词：黑色素瘤分化相关基因5(MDA5) 接头蛋白 磷酸化 Ⅰ型干扰素 

分 类 号：S852.4[农业科学—基础兽医学]