利用酵母双杂交系统筛选水稻组蛋白去乙酰化酶HDA705的互作蛋白  

Screening of Proteins Interacting with Histone Deacetylase HDA705 in Rice(Oryza sativa) by Yeast Two Hybrid System

作　　者：李明智[1,2] 赵金会[1] 刘勋成[1] 张建霞[1] 段俊[1] 

机构地区：[1]中国科学院华南植物园,广州510650 [2]中国科学院大学,北京100049

出　　处：《热带亚热带植物学报》2015年第1期43-50,共8页Journal of Tropical and Subtropical Botany

基　　金：广东省科技计划项目(2011A020102008)资助

摘　　要：为了解水稻(Oryza sativa)组蛋白去乙酰化酶HDA705的生物学功能,构建了HDA705酵母双杂交诱饵表达载体与双杂交文库,并筛选了与HDA705相互作用的蛋白。结果表明,HDA705的诱饵载体无自激活活性且对酵母无毒性作用,文库的滴度也适合常规的酵母双杂交文库筛选。通过对酵母双杂交文库的筛选,共获得了164个阳性克隆,经DNA测序分析,这些克隆编码47个可能与HDA705相互作用的蛋白,其中包括3个在逆境响应或激素信号转导过程中起到重要作用的(辅)转录因子、6个参与光合作用的叶绿体蛋白、1个含有R3H结构域的蛋白以及22种酶类等。这为进一步研究HDA705的生物学功能提供了重要的线索。In order to understand the biological function of histone deacetylase HDA705 in rice, the yeast twohybridbait vector of HDA705 and the cDNA library were constructed and the interaction partners of HDA705were screened. The results showed that HDA705 bait vector showed no self-activating and no toxicity to yeastcells. Furthermore, the titer of cDNA library was suitable for normal yeast two-hybrid screening assay. Therewere 164 positive clones by yeast two-hybrid screening. DNA sequencing analysis indicated that these positiveclones encoded 47 proteins which may interact with HDA705. These proteins included 3 transcription factors （ortranscription co-factors） which play key roles in stress responses and hormone signaling pathways, 6 chloroplastlocated proteins which were involved in photosynthesis, 1 protein containing the R3H domain structure, and 22enzymes in plant, etc. These may provide important cues to further study the biological function of HDA705 in rice.

关 键 词：水稻 组蛋白去乙酰化酶 酵母双杂交 互作蛋白 

分 类 号：Q946.5[生物学—植物学]