HLPC法测定感冒止咳颗粒中葛根素的含量  

Determination of puerarin in Ganmao Zhike Granules by HPLC

作　　者：李媛 李强 

机构地区：[1]东营市食品药品检验中心,山东东营257091

出　　处：《药学研究》2015年第1期13-15,共3页Journal of Pharmaceutical Research

摘　　要：目的采用高效液相色谱法测定感冒止咳颗粒中葛根素的含量.方法Agilent Eclipse XDB-C18柱（4.6mm×200mm,5μm）;以甲醇-水溶液（28∶72）为流动相;检测波长为250nm,流速为1.0mL·min^-1,柱温：35℃.理论板数按葛根素峰计算应不低于4000.结果葛根素与其相邻杂质峰能完全分离,葛根素进样量在0.08822～0.4411μg之间与峰面积呈良好的线性关系,相关系数R^2 =1.0000,平均加样回收率为97.66%,RSD=0.8017%（n=6）.结论本方法操作简便,精密度好,结果准确可靠,可用于感冒止咳颗粒的质量控制.Objective To establish a method for the determination of puerarin in Ganmao Zhike Granules by HPLC. Methods The C18column(4. 6 mm × 200 mm,5 μm) was adopted with the mobile phase of methanol-water(28∶72) at the flow rate o f 1. 0 m L·min-1,and the detection wavelength was 250 nm. Theoretical plate number should not be less than 4 000 according to the puerarin peak. Results The date showed that this method could clearly separate puerarin peak from its nearby impurity peaks,with a better linear range of 0. 008 82 ~ 0. 441 1 μg(R2= 1. 000 0),and the average recovery was 97. 66% and the RSD was 0. 801 7%(n = 6). Conclusion This method was simple,accurate and reliable,so it can be used for the quality control of Ganmao Zhike Granules.

关 键 词：感冒止咳颗粒 葛根素 含量测定 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]