疏浚泥悬浮液和浸出液对三疣梭子蟹大眼幼体的急性毒性及热应激蛋白70基因表达的影响  

Acute Toxicity of Suspension and Lixivium From Dredged Sludge to Megalopa of Portunus trituberculatus and Effect of the Substances on HSP70 Gene Expression in the Megalopa

作　　者：蒋玫[1] 李磊[1] 沈新强[1] 吴庆元[2] 牛俊翔[2] 许高鹏[2] 

机构地区：[1]中国水产科学研究院东海水产研究所,上海200090 [2]上海海洋大学海洋科学学院,上海201306

出　　处：《生态与农村环境学报》2015年第1期118-123,共6页Journal of Ecology and Rural Environment

基　　金：上海市科学技术委员会项目(12231203403);国家重点基础研究发展计划(G2010CB429005)

摘　　要：研究了洋山航道工程疏浚泥悬浮液和浸出液对三疣梭子蟹(Portunus trituberculatus)大眼幼体的毒害效应,结果表明:(1)大眼幼体死亡率随着悬浮液和浸出液配比浓度的增大而逐渐增加。浸出液对大眼幼体的96 h半致死配比浓度φ(LC50)为1 294.66 m L·L-1,安全配比浓度φ(SC)为129.466 m L·L-1;悬浮液对大眼幼体的96hφ(LC50)为996.17 m L·L-1,φ(SC)为99.617 m L·L-1。(2)大眼幼体的蜕皮率随着悬浮液和浸出液配比浓度的增加而逐渐降低。同体积配比浓度下,浸出液处理组蜕皮率略高于悬浮液处理组。悬浮液抑制三疣梭子蟹大眼幼体的蜕壳,而浸出液抑制新壳的硬化。(3)浸出液和悬浮液胁迫24 h后,大眼幼体肌肉中HSP70表达量迅速升高,浸出液和悬浮液最高配比浓度组表达量显著高于对照组(P<0.05);72 h各处理组HSP70表达量均达到最高值;96 h后各处理组表达量有所降低,最高配比浓度组HSP70表达量降幅明显(P<0.05),对照组表达量仍继续上升。Toxic effect of suspension and lixivium from the dredged sludge of the Yangshan channel project on megalopa of Portunus trituberculatus was evaluated, through a laboratory simulation experiment. Results show that ( 1) mortality of the megalopa increased gradually with concentration of the suspension and lixivium, and 96 h LC50 and SC of the lixivium to megalopa of Portunus trituberculatus was 1 294?66 and 129?466 mL·L-1, respectively, and of the suspension, 996?17 and 99?617 mL·L-1 , respectively;( 2) molting rate of the megalopa decreased with increasing concentration of the sus?pension and lixivium;it was slightly higher in the group of lixivium treatments than in the group of suspension treatments group when they were the same in volume concentration;the suspension inhibited molting of megalopa, while the lixivium inhibited hardening of new shell;( 3) After 24 hours of exposure to the suspension and lixivium, the expression of HSP70 in the muscle of megalopa increased rapidly; after 72 hours, it peaked in all the treatments; and after 96 hours, it de?creased to a varying extent in all the treatments and the most significantly in the treatment the highest in concentration;while in the control it kept on rising.

关 键 词：三疣梭子蟹 疏浚泥 蜕皮率 HSP70 

分 类 号：S917[农业科学—水产科学]