胶体金免疫试纸法快速测定雌三醇  

Study of Colloidal Gold Immuno-chromatographic Assay Stick for Detection of Estriol

作　　者：邱凌[1] 王佳妮[1] 廖旭[1] 洪子肖[1] 张娴[1] 

机构地区：[1]中国科学院城市环境研究所,城市环境与健康重点实验室,分析测试中心,福建厦门361021

出　　处：《厦门大学学报（自然科学版）》2015年第1期47-51,共5页Journal of Xiamen University：Natural Science

基　　金：福建省自然科学基金(2011J0102)

摘　　要：雌三醇(E3)在临床诊断、日用化工和畜牧业等领域具有广泛应用,同时它也是一种环境污染物,对E3的检测具有科学和应用意义.本研究采用竞争性免疫层析技术,以E3单克隆抗体-纳米胶体金复合物作为金标抗体,以E3偶联抗原为检测线(T线)、以羊抗鼠抗抗体为控制线(C线),通过条件的优化,制备出检测E3的胶体金试纸条.当试纸条C线和T线同时显色时,结果为阴性;当试纸条仅C线显色时,结果为阳性.所得检测试纸条测加标水样的最低检测限为100μg/L,测加标奶样的最低检测限为100μg/L,反应时间为10min,与雌酮、雌二醇、己烷雌酚等环境雌激素无交叉反应,具有良好的选择性.Estriol（E3）is useful in clinical diagnosis,daily chemical and animal husbandry,as well as an environmental pollutant,therefore,the detection of E3 has significance in both science and applications.In this study,we developed a competitive immuno-chromatographic assay stick to test E3.Nano-colloidal gold particles were prepared and labled to an anti-estriol monoclonal antibody.E3 was conjugated to ovalbumin（OVA）and dispersed on a nitroclonal（NC）membrane as the test line（T line）.Goat anti-mouse IgG was dispersed on the NC membrane as the control line（C line）.Through optimizing,the assay stick was developed for E3 detection.Two colored bands in the test line and control line indicated a negative result.A colored band in the control line indicated a positive result.The minimum detectable concentration of E3 could reach up to 100μg/L.The test could be completed in 10 minutes.The assay stick didn′t have the cross reactivity to estrone,estradiol and hexoestrolum,indicating the high specificity of this E3 detection assay.

关 键 词：雌三醇 胶体金 免疫层析 试纸条 

分 类 号：Q57[生物学—生物化学]