中国鲎脑神经节5-羟色胺免疫组化的定位观察  

Immunohistochemical Study on the Distribution of 5-hydroxytryptamine in the Brain of Tachypleus tridentatus

作　　者：李林明[1] 黄辉洋[1,2] 叶海辉[1] 李少菁[1] 

机构地区：[1]厦门大学海洋与地球学院,福建厦门361102 [2]福建农林大学动物科学学院

出　　处：《厦门大学学报（自然科学版）》2015年第1期64-68,共5页Journal of Xiamen University：Natural Science

基　　金：国家自然科学基金项目(41076081,31272632)

摘　　要：中国鲎(Tachypleus tridentatus)是一种具有特殊研究意义的海洋动物.本研究应用免疫组织化学方法对中国鲎脑神经节中5-羟色胺(5-HT)的分布进行了定位观察,发现5-HT免疫阳性主要分布在视神经层和视神经髓的神经纤维,中间体以及蕈形体周边的细胞群中,而蕈形体中未发现免疫阳性.经比较,中国鲎5-HT的分布与美洲鲎(Limulus polyphemus)相似,而与昆虫、甲壳类存在一些区别.本研究可为中国鲎的神经生物学提供基础资料.The horseshoe crab Tachypleus tridentatus is an important marine species in science study.The distribution of 5-hydroxytryptamine（5-HT）of neurons and fibers in the brain of T.tridentatus was investigated by immunohistochemistry.The 5-HT immunoreactivity was mainly localized in the laminar neuropil,medullar neuropil,central body neuropil,dorsal media group and the neurons around the corpus pedunculatum,but the corpus pedunculatum showed the negative immunoreactivity.The distribution of 5-HT in T.tridentatus is similar to another horseshoe crab species,Limulus polyphemus,and different from insects and crustaceans.This study supplies some fundamental data for neurobiology in T.tridentatus.

关 键 词：中国鲎 脑神经节 5-羟色胺 免疫组化 

分 类 号：Q426[生物学—神经生物学] S917.4[生物学—生理学]