破血化瘀填精补髓方剂对脑出血大鼠模型神经营养因子BDNF表达的影响  

Effect of Poxuehuayutianjingbusui Decoction in the Expression of Neurotrophic Factor BDNF in Experimental ICH Rats

作　　者：张轶丹[1] 赵建军[1] 金曦[1] 王泽玉[1] 李霞[1] 于兰[1] 郑鹏[1] 崔雅斌[1] 荣春书[1] 王庆伟[1] 温泉[1] 

机构地区：[1]长春中医药大学附属医院,吉林长春130021

出　　处：《中国中医急症》2015年第1期1-3,23,共4页Journal of Emergency in Traditional Chinese Medicine

基　　金：国家中医药管理局中风病破血化瘀重点研究室项目

摘　　要：目的观察破血化瘀填精补髓方剂对脑出血大鼠脑出血后不同时间点脑源性神经生长因子(BDNF)表达的影响。方法选用Wistar大鼠复制脑出血大鼠模型,观察不同剂量破血化瘀填精补髓方剂改善脑出血大鼠神经功能缺损的作用,及出血后不同时间点神经营养因子BDNF表达情况。结果在给药第1、3、7日,脑出血损伤增加损伤区域内的BDNF阳性细胞数量;而且在损伤的第3日因损伤刺激出现的BDNF阳性细胞数量达到高峰,第7日已经开始回落。应用破血化瘀填精补髓方剂干预后,可以延长BDNF阳性细胞数量达到高峰的时间。结论破血化瘀填精补髓方剂能明显改善脑出血大鼠神经功能缺损;其作用机制可能与上调脑出血血肿周围损伤组织中BDNF表达有关。Objective： To investigate the Poxuehuayutianjingbusui Decoction on the expression of neurotrophic factor BDNF in rats with cerebral hemorrhage. Methods： Wistar,establishment of rat models with cerebral hemorrhage broken blood stasis,were used the Poxuehuayutianjingbusui Decoction. They were observed the improvment of hemorrhage rat neural function defect effect and the expression of bleeding at different time points after the neurotrophic factor BDNF. Results： In the administration of the 1 st,3 rd,7 th day,cerebral hemorrhage injury increased damage within the region the number of BDNF positive cells;and on the third day injury due to damage to stimulate the emergence of the number of BDNF positive cells reached the peak,seventh days have begun to fall.And the application of Poxuehuayutianjingbusui Decoction intervention can prolong the time of the amount of BDNF positive cells reached the peak of the it. Conclusion： Poxuehuayutianjingbusui Decoction can significantly improve the neurological function of rats with intracerebral hemorrhage related defects;the expression of BDNF damage tissue around the hematoma and its mechanism may be related to the upregulation of cerebral hemorrhage.

关 键 词：脑出血 神经营养因子 破血化瘀填精补髓方剂 

分 类 号：R285.5[医药卫生—中药学]