小檗碱通过调节凋亡相关基因的表达抑制金黄色葡萄球菌诱导的ECV-304细胞凋亡  

Berberine Inhibiting Staphylococcus Aureus-Induced Apoptosis in ECV-304 Cells via Regulating Bcl-2,Bax and Caspase-3 Expressions

作　　者：裴德翠 熊传银 毕爱芬 胡汉斌 

机构地区：[1]广州市中西医结合医院检验科,广东广州510800

出　　处：《辽宁中医杂志》2015年第1期35-37,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：广东省中医药局项目(20121029)

摘　　要：目的:研究小檗碱在金黄色葡萄球菌(S.aureus)诱导ECV-304细胞内凋亡相关基因Bcl-2、Bax和Caspase-3表达的调节作用,探讨其抑制金黄色葡萄球菌诱导ECV-304细胞凋亡的机制。方法:小檗碱(Berberine,BBR)预处理ECV-304细胞2h后,用1∶100感染复数的S.aureus感染细胞,采用半定量RT-PCR及Western-blot法检测金黄色葡萄球菌感染后ECV-304细胞内Bcl-2、Bax和Caspase-3的mRNA及蛋白表达水平。结果:小檗碱预处理ECV-304后再感染S.aureus可显著性增加凋亡相关细胞因子Bcl-2 mRNA水平及蛋白表达量,而Bax和Caspase-3表达下调。结论:小檗碱可以通过调节凋亡相关基因Bcl-2、Bax和Caspase-3的表达抑制S.aureus诱导的ECV-304细胞凋亡,本实验结果为临床治疗S.aureus感染提供理论依据。Objective： To study the protection of berberine against ECV-304 apoptosis induced by Staphylococcus aureus（ S.aureus） via regulating Bcl-2,Bax and Caspase-3 expressions and to discuss the mechanism that berberine inhibits apoptosis of ECV-304 cells induced by S. aureus. Methods： ECV-304 was pretreated with 128 μg / m L BBR for 2 h,and then S aureus（ 1∶100） was added. The expression levels of apoptosis related genes were detected with semi-quantity RT-PCR and Western-blot. Results： Pretreatment of BBR helped to survive in S aureus-induced ECV-304. BBR significantly increased the expression levels of Bcl-2 and decreased that of Bax and Caspase-3 after staphylococcus aureus infection. Conclusion： Berberine inhibits staphylococcus aureus-induced apoptosis in ECV-304 cells via regulating Bcl-2,Bax and Caspase-3 expressions. This study provides evidence for clinical treatment.

关 键 词：小檗碱 金黄色葡萄球菌 ECV-304 凋亡 

分 类 号：R285.5[医药卫生—中药学]