双夏汤不同配比对夏枯草中迷迭香酸提取率的影响  

Effect of Different Compatibilities of Shuangxia Decoction on Extraction Yield of Rosmarinic Acid in Prunellae

作　　者：张志千[1] 汪文琪[1] 张俊义[1] 孙毅坤[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《辽宁中医杂志》2015年第1期138-140,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然基金基于受体学说的双夏汤治疗失眠的物质基础与作用机制研究(81173521)

摘　　要：目的：研究半夏、夏枯草药对配伍后对夏枯草中迷迭香酸提取率的影响。方法：采用Thermo Scientific-C18色谱柱（250 mm×4.6 mm,5μm）,以甲醇-1%甲酸（30∶70）为流动相,流速为1 m L/min,检测波长为330 nm,柱温40℃。结果：迷迭香酸进样量在0.1-0.5μg范围内有良好的线性关系（r=0.9998）,平均回收率为101.1%（RSD=1.71%,n=6）。双夏汤配伍后,半夏与夏枯草比例大于1.5∶1时迷迭香酸提取率降低,小于1.5∶1时迷迭香酸提取率升高。结论：双夏汤不同配伍中半夏的含量影响夏枯草中迷迭香酸的提取率。Objective： To study the effect of pinellia combined with prunellae vulgaris in different ratios on the extraction yield of rosmarinic acid. Methods： A Thermo Scientific-C18 column（ 250 mm × 4. 6 mm,5 μm） was used. The mobile phase was the mixture of methanol-1% formic acid（ 30∶ 70）. The flow rate was 1 m L / min and the UV detection wavelength was 330 nm. The column temperature was 40 ℃. Results： The linear range of rosmarinic acid was 0. 1 μg-0. 5 μg（ r = 0. 9998） and the average recovery rate of rosmarinic acid was 101. 1%（ RSD = 1. 71%,n = 6）. Compared with prunellae,the extraction yield of rosmarinic acid was reduced when pinellia and prunellae ratio was greater than 1. 5 ∶ 1. And the extraction yield of rosmarinic acid was increased when pinellia and prunellae ratio was less than 1. 5∶ 1. Conclusion： Pinellia influents the extraction rate of rosmarinic acid in Shuangxia Decoction.

关 键 词：夏枯草 迷迭香酸 双夏汤 HPLC 含量测定 

分 类 号：R284.2[医药卫生—中药学]