采用紫外分光光度法评价石油烃降解菌的降解能力  

Evaluation of degrading ability of petroleum hydrocarbon degrading bacteria by ultraviolet spectrophotometry

作　　者：蒋瑞萍[1] 孙丽丽[1] 解开治[1,2] 卢钰升[1] 李夏[1] 顾文杰[1] 李文英[1] 徐培智[1] 

机构地区：[1]广东省农业科学院农业资源与环境研究所,农业部南方植物营养与肥料重点实验室,广东省养分资源循环利用与耕地保育重点实验室,广东广州510640 [2]美国加州大学伯克利分校植物与微生物学系,美国旧金山伯克利94720

出　　处：《石化技术与应用》2015年第1期70-74,共5页Petrochemical Technology & Application

基　　金：广东省农业科学院"科技创新人才出国(境)培养计划"项目(项目名称:分子生物技术在环境工程微生物领域中的应用研究;项目编号:201314);美国加州大学伯克利分校校企合作项目(项目名称:加利福尼亚州海湾石油烃污染的生物修复;项目编号:2013CAPMB012)

摘　　要：为克服摇瓶培养过程中石油烃降解菌代谢产物对紫外分光光度法比色的干扰,建立了离心前处理—超声波萃取—紫外分光光度法,验证并评价了石油烃降解菌Acinetobacter sp RP-1的降解能力。结果表明,在254 nm最佳吸收波长下,以沸程为30~60℃的石油醚为萃取剂,在石油烃质量浓度为5~300 mg/L范围内,吸光度与石油烃浓度间符合比尔定律关系,工作曲线的相关系数为0.999 8;采用离心前处理和超声波萃取处理样品,石油烃的平均回收率为98.9%,相对标准偏差为1.78%,最低检出限为0.713 mg/L;可检测出无机盐培养基中低至0.001%的残余烃。In order to overcome the interference of petroleum hydrocarbon degrading bacterial metabo- lites with colorimetrie determination of ultraviolet spectrophotometry during shake - flask culture, cen- trifugal pretreatment - ultrasonic extraction - ultravi- olet spectrophotometry was established and the de- grading ability of petroleum hydrocarbon degrading bacteria Acinetobacter sp RP - 1 was verified and e- valuated. The results showed that under the condi- tion of the optimum absorption wavelength 254 nm, boiling range of petroleum ether extraction agent 0 - 60℃,the mass concentration of petroleum carbon in the range of 5 -300mg/L, the absorbency and the concentration of petroleum hydrocarbon was in ac- cordance with Beer＇s law, the correlation coefficient of working curve was 0. 999 8 ; for the samples trea- ted by centrifugal pretreatment and ultrasonic extraction, the average recovery rate of petroleum hydrocarbon was 98.9% , the relative standard deviation 1.78% , the lowest detection limit 0. 713 mg/L and the residual hydrocarbon concentration as low as 0. 001% in the inorganic salt medium could be detected.

关 键 词：紫外分光光度法 石油烃降解菌 萃取 比色测定 

分 类 号：X832[环境科学与工程—环境工程]