牛精液新型冷冻保护剂的研究  

Study on New Kinds of Cryoprotectants for Bovine Sperm

作　　者：桑国俊[1] 邱忠玉[1] 郭海龙[1] 王珂[1] 容维中[1] 

机构地区：[1]甘肃省畜牧兽医研究所,甘肃平凉744000

出　　处：《畜牧兽医杂志》2015年第1期22-27,共6页Journal of Animal Science and Veterinary Medicine

摘　　要：通过对不同浓度的甘油和乙二醇对牛精液冷冻保存效果的测定,探讨冷冻保护剂乙二醇与传统冷冻保护剂甘油的冷冻效果,并通过对葡萄糖和卵黄及Tris等成分的调整进一步分析了主要营养成分和缓冲体系对牛精液冷冻保存的影响程度。试验表明:1GL和EG对牛精子均有良好的冷冻保护能力,解冻后6h的活力GL组中以GL1.2和GL1.4的活力最高,均为0.50;EG组中以EG0.7的活力最高,达到0.53,高于0.35以上的国标。2其他营养成分的变化对GL和EG保护精子的效果有一定影响,特别是卵黄的作用比较明显;3在同等条件下,GL对精子的冷冻保护效果优于EG,EG组的活力衰竭快于GL组。Ethylene glycol （EG） and Glycerol （GL） as eryoproteetants had increased the thawing sperm motility for bovine frozen semen of wheat straw. The results showed that, the proper density with high motility were GL1.2 and GL1.4 as the re- sults of all the treatments of GL. And the group of EG was EGO. 7. The second was that chicken yolk was very important for bovine sperm frozen preservation. The third was that the efficiency of thawing motility of GL was better than the EG group＇s.

关 键 词：甘油 乙二醇 牛细管冻精 精子活力 

分 类 号：S814.3[农业科学—畜牧学]